LOCUS       BBa_K362019             1869 bp    DNA     linear       07-May-2024
DEFINITION  sufA promoter - RBS - RFP - Term - Term
ACCESSION   BBa_K362019
VERSION     BBa_K362019.1  
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /label=BBa_K362005
     misc_feature    1013..1016
                     /label=conserved
     RBS             1009..1020
                     /label=BBa_B0034
     CDS             1027..1701
                     /label=mrfp1
     misc_feature    1708..1732
                     /label=Help:Barcodes
     CDS             1027..1707
                     /label=BBa_E1010
     stem_loop       1752..1795
                     /label=stem_loop
     misc_feature    1741..1820
                     /label=BBa_B0010
     terminator      1741..1820
                     /label=BBa_B0010
     stem_loop       1836..1855
                     /label=T7 TE
     misc_feature    1862..1862
                     /label=stop
     polyA_site      1856..1869
                     /label=polya
     misc_feature    1829..1869
                     /label=BBa_B0012
     terminator      1829..1869
                     /label=BBa_B0012
     misc_feature    1009..1869
                     /label=BBa_I13507
ORIGIN
        1 ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggcgtatgc
       61 aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa
      121 gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa
      181 agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag
      241 gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt
      301 gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa
      361 caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa
      421 atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca
      481 aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc
      541 gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt
      601 tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc
      661 gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc
      721 tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt
      781 ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat
      841 tcttagcgtg cctgttaacc cacacatcag ggtctatgct tattaaaagc aggcaaaaag
      901 catgttttac ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac
      961 atgctgttat acgctgaaag cgatgaagtg aggtaaatcg tactagagaa agaggagaaa
     1021 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     1081 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     1141 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     1201 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     1261 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1321 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1381 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     1441 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     1501 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     1561 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     1621 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     1681 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
     1741 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1801 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1861 gcgtttata
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