LOCUS       BBa_K362020             1503 bp    DNA     linear       07-May-2024
DEFINITION  yaiA promoter - RBS - RFP - Term - Term
ACCESSION   BBa_K362020
VERSION     BBa_K362020.1  
FEATURES             Location/Qualifiers
     promoter        1..634
                     /label=BBa_K362006
     misc_feature    647..650
                     /label=conserved
     RBS             643..654
                     /label=BBa_B0034
     CDS             661..1335
                     /label=mrfp1
     misc_feature    1342..1366
                     /label=Help:Barcodes
     CDS             661..1341
                     /label=BBa_E1010
     stem_loop       1386..1429
                     /label=stem_loop
     misc_feature    1375..1454
                     /label=BBa_B0010
     terminator      1375..1454
                     /label=BBa_B0010
     stem_loop       1470..1489
                     /label=T7 TE
     misc_feature    1496..1496
                     /label=stop
     polyA_site      1490..1503
                     /label=polya
     misc_feature    1463..1503
                     /label=BBa_B0012
     terminator      1463..1503
                     /label=BBa_B0012
     misc_feature    643..1503
                     /label=BBa_I13507
ORIGIN
        1 caccgcagat atacctttgg ctgctgaagc catcgagaaa ggcgctgcgg cgcttaatcc
       61 gcgcggcgaa cgttacacgc cagcgaccat tcgtgagcgc ctgacgatgc gcgattttat
      121 ggatacctta cgtgccagtg ggatccagac cggcggacca gatagccttt cacaacgtga
      181 ccgccaggcc tttgccgcgg agctggagaa gtggtggctg gaagtgcaac gtagtcgtgg
      241 ctaaatgtaa tttattattt acacttcatt cttgaatatt tattggtata gtaaggggtg
      301 tattgagatt ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga
      361 tggtatgatc gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa
      421 cccacgatga cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc
      481 ggaatggccc ttgccgattc gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa
      541 tcacagctca atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc
      601 gccagagaaa cggcggcgct ggaagcggta actgtactag agaaagagga gaaatactag
      661 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
      721 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      781 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      841 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      901 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      961 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
     1021 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
     1081 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
     1141 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
     1201 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
     1261 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
     1321 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
     1381 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1441 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1501 ata
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