LOCUS       BBa_K362059             1266 bp    DNA     linear       04-May-2024
DEFINITION  acrAB promoter - RBS - GFP - Term - Term
ACCESSION   BBa_K362059
VERSION     BBa_K362059.1  
FEATURES             Location/Qualifiers
     promoter        1..327
                     /label=BBa_K362000
     misc_feature    336..355
                     /label=BBa_J54033
     misc_feature    336..341
                     /label=SalI
     misc_feature    350..355
                     /label=BamHI
     misc_feature    362..381
                     /label=BBa_J54025
     misc_feature    362..367
                     /label=BglII
     misc_feature    376..381
                     /label=MulI
     RBS             384..399
                     /label=RBS-1\Strong
     misc_feature    384..399
                     /label=BBa_B0030
     RBS             391..397
                     /label=RBS
     misc_feature    406..1125
                     /label=BBa_E0040
     misc_feature    1135..1214
                     /label=BBa_B0010
     misc_feature    1222..1262
                     /label=BBa_B0012
     stem_loop       1146..1189
                     /label=stem_loop
     stem_loop       1234..1253
                     /label=T7 TE
     misc_feature    336..1266
                     /label=BBa_J54103
     misc_feature    336..340
                     /label=SalI
     misc_feature    336..354
                     /label=BBa_J54033
     misc_feature    350..354
                     /label=BamHI
     misc_feature    363..367
                     /label=BglII
     misc_feature    363..381
                     /label=BBa_J54025
     misc_feature    376..381
                     /label=MulI
     RBS             390..404
                     /label=RBS-1\Strong
     misc_feature    390..404
                     /label=BBa_B0030
     RBS             397..401
                     /label=RBS
     misc_feature    411..1130
                     /label=BBa_E0040
     misc_feature    1139..1218
                     /label=BBa_B0010
     stem_loop       1150..1193
                     /label=stem_loop
     misc_feature    1227..1267
                     /label=BBa_B0012
     stem_loop       1234..1253
                     /label=T7 TE
     polyA_site      1254..1267
                     /label=polya
     misc_feature    1260..1260
                     /label=stop
     misc_feature    336..1266
                     /label=BBa_J54103
     misc_feature    336..1266
                     /label=BBa_K121013
ORIGIN
        1 gatctcactg aacaaatccg acttgtcttt aaaatgccac tagattgcac cgcgcgtaac
       61 gccagctgct ttttgcaatc tcgcccagcg aggtggatga taccccctgc tgtgagaaaa
      121 gacgtagagc cacatcgagg atgtgttggc gcgtttcttg cgcttcttgt ttggtttttc
      181 gtgccatatg ttcgtgaatt tacaggcgtt agatttacat acatttgtga atgtatgtac
      241 catagcacga cgataatata aacgcagcaa tgggtttatt aacttttgac cattgaccaa
      301 tttgaaatcg gacactcgag gtttacatac tagaggtcga ctgacgactg gatcctatca
      361 agatctgtcg gataacgcgt tactagagat taaagaggag aaatactaga tgcgtaaagg
      421 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      481 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      541 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      601 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      661 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      721 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      781 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      841 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      901 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      961 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     1021 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     1081 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca
     1141 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1201 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1261 tttata
//