LOCUS       BBa_K362060             1439 bp    DNA     linear       07-May-2024
DEFINITION  dps promoter - RBS - GFP - Term - Term
ACCESSION   BBa_K362060
VERSION     BBa_K362060.1  
FEATURES             Location/Qualifiers
     promoter        1..500
                     /label=BBa_K362002
     misc_feature    509..528
                     /label=BBa_J54033
     misc_feature    509..514
                     /label=SalI
     misc_feature    523..528
                     /label=BamHI
     misc_feature    535..554
                     /label=BBa_J54025
     misc_feature    535..540
                     /label=BglII
     misc_feature    549..554
                     /label=MulI
     RBS             557..572
                     /label=RBS-1\Strong
     misc_feature    557..572
                     /label=BBa_B0030
     RBS             564..570
                     /label=RBS
     misc_feature    579..1298
                     /label=BBa_E0040
     misc_feature    1308..1387
                     /label=BBa_B0010
     misc_feature    1395..1435
                     /label=BBa_B0012
     stem_loop       1319..1362
                     /label=stem_loop
     stem_loop       1407..1426
                     /label=T7 TE
     misc_feature    509..1439
                     /label=BBa_J54103
     misc_feature    509..513
                     /label=SalI
     misc_feature    509..527
                     /label=BBa_J54033
     misc_feature    523..527
                     /label=BamHI
     misc_feature    536..540
                     /label=BglII
     misc_feature    536..554
                     /label=BBa_J54025
     misc_feature    549..554
                     /label=MulI
     RBS             563..577
                     /label=RBS-1\Strong
     misc_feature    563..577
                     /label=BBa_B0030
     RBS             570..574
                     /label=RBS
     misc_feature    584..1303
                     /label=BBa_E0040
     misc_feature    1312..1391
                     /label=BBa_B0010
     stem_loop       1323..1366
                     /label=stem_loop
     misc_feature    1400..1440
                     /label=BBa_B0012
     stem_loop       1407..1426
                     /label=T7 TE
     polyA_site      1427..1440
                     /label=polya
     misc_feature    1433..1433
                     /label=stop
     misc_feature    509..1439
                     /label=BBa_J54103
     misc_feature    509..1439
                     /label=BBa_K121013
ORIGIN
        1 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
       61 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tactggcgct
      121 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
      181 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
      241 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
      301 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
      361 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
      421 ccagaatagc ggaacacata gccggtgcta tacttaatct cgttaattac tgggacataa
      481 catcaagagg atatgaaatt tactagaggt cgactgacga ctggatccta tcaagatctg
      541 tcggataacg cgttactaga gattaaagag gagaaatact agatgcgtaa aggagaagaa
      601 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      661 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      721 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      781 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      841 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      901 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      961 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     1021 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     1081 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     1141 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     1201 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     1261 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
     1321 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1381 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//