LOCUS       BBa_K362063             1573 bp    DNA     linear       07-May-2024
DEFINITION  yaiA promoter - RBS - GFP - Term - Term
ACCESSION   BBa_K362063
VERSION     BBa_K362063.1  
FEATURES             Location/Qualifiers
     promoter        1..634
                     /label=BBa_K362006
     misc_feature    643..662
                     /label=BBa_J54033
     misc_feature    643..648
                     /label=SalI
     misc_feature    657..662
                     /label=BamHI
     misc_feature    669..688
                     /label=BBa_J54025
     misc_feature    669..674
                     /label=BglII
     misc_feature    683..688
                     /label=MulI
     RBS             691..706
                     /label=RBS-1\Strong
     misc_feature    691..706
                     /label=BBa_B0030
     RBS             698..704
                     /label=RBS
     misc_feature    713..1432
                     /label=BBa_E0040
     misc_feature    1442..1521
                     /label=BBa_B0010
     misc_feature    1529..1569
                     /label=BBa_B0012
     stem_loop       1453..1496
                     /label=stem_loop
     stem_loop       1541..1560
                     /label=T7 TE
     misc_feature    643..1573
                     /label=BBa_J54103
     misc_feature    643..647
                     /label=SalI
     misc_feature    643..661
                     /label=BBa_J54033
     misc_feature    657..661
                     /label=BamHI
     misc_feature    670..674
                     /label=BglII
     misc_feature    670..688
                     /label=BBa_J54025
     misc_feature    683..688
                     /label=MulI
     RBS             697..711
                     /label=RBS-1\Strong
     misc_feature    697..711
                     /label=BBa_B0030
     RBS             704..708
                     /label=RBS
     misc_feature    718..1437
                     /label=BBa_E0040
     misc_feature    1446..1525
                     /label=BBa_B0010
     stem_loop       1457..1500
                     /label=stem_loop
     misc_feature    1534..1574
                     /label=BBa_B0012
     stem_loop       1541..1560
                     /label=T7 TE
     polyA_site      1561..1574
                     /label=polya
     misc_feature    1567..1567
                     /label=stop
     misc_feature    643..1573
                     /label=BBa_J54103
     misc_feature    643..1573
                     /label=BBa_K121013
ORIGIN
        1 caccgcagat atacctttgg ctgctgaagc catcgagaaa ggcgctgcgg cgcttaatcc
       61 gcgcggcgaa cgttacacgc cagcgaccat tcgtgagcgc ctgacgatgc gcgattttat
      121 ggatacctta cgtgccagtg ggatccagac cggcggacca gatagccttt cacaacgtga
      181 ccgccaggcc tttgccgcgg agctggagaa gtggtggctg gaagtgcaac gtagtcgtgg
      241 ctaaatgtaa tttattattt acacttcatt cttgaatatt tattggtata gtaaggggtg
      301 tattgagatt ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga
      361 tggtatgatc gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa
      421 cccacgatga cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc
      481 ggaatggccc ttgccgattc gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa
      541 tcacagctca atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc
      601 gccagagaaa cggcggcgct ggaagcggta actgtactag aggtcgactg acgactggat
      661 cctatcaaga tctgtcggat aacgcgttac tagagattaa agaggagaaa tactagatgc
      721 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      781 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      841 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      901 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      961 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1021 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1081 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1141 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1201 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1261 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1321 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1381 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1441 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1501 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1561 ttctgcgttt ata
//