LOCUS BBa_K376034 3902 bp DNA linear 14-Jun-2024 DEFINITION Oxygen Sensing AFP Generator+LuxI+Tet Inverter+ Lambda Repressor+LuxR Generator ACCESSION BBa_K376034 VERSION BBa_K376034.1 FEATURES Location/Qualifiers promoter 1..79 /label=BBa_K376002 RBS 88..113 /label=Super RBS CDS 119..535 /label=Anaerobic Flourescent Protein misc_feature 88..535 /label=BBa_K376004 misc_feature 1..535 /label=BBa_K376006 misc_feature 548..551 /label=conserved RBS 544..555 /label=BBa_B0034 misc_feature 1141..1172 /label=LVA CDS 562..1140 /label=luxI misc_feature 562..1179 /label=BBa_C0061 misc_feature 1180..1204 /label=Help:Barcodes CDS 562..1179 /label=BBa_C0061 misc_feature 544..1204 /label=BBa_C0261 misc_feature 1217..1220 /label=conserved RBS 1213..1224 /label=BBa_B0034 CDS 1234..1850 /label=tetR misc_feature 1851..1884 /label=SsrA misc_feature 1891..1915 /label=Help:Barcodes CDS 1231..1890 /label=BBa_C0040 stem_loop 1935..1978 /label=stem_loop misc_feature 1924..2003 /label=BBa_B0010 terminator 1924..2003 /label=BBa_B0010 stem_loop 2019..2038 /label=T7 TE misc_feature 2045..2045 /label=stop polyA_site 2039..2052 /label=polya misc_feature 2012..2052 /label=BBa_B0012 terminator 2012..2052 /label=BBa_B0012 misc_feature 2061..2079 /label=TetR 1 misc_feature 2061..2114 /label=BBa_R0040 promoter 2080..2085 /label=-35 misc_feature 2086..2104 /label=TetR 2 promoter 2103..2108 /label=-10 promoter 2061..2114 /label=BBa_R0040 misc_feature 1213..2114 /label=BBa_Q04400 misc_feature 2127..2130 /label=conserved RBS 2123..2134 /label=BBa_B0034 CDS 2144..2851 /label=cI lambda misc_feature 2852..2884 /label=LVA misc_feature 2891..2915 /label=Help:Barcodes CDS 2141..2890 /label=BBa_C0051 stem_loop 2935..2978 /label=stem_loop misc_feature 2924..3003 /label=BBa_B0010 terminator 2924..3003 /label=BBa_B0010 stem_loop 3019..3038 /label=T7 TE misc_feature 3045..3045 /label=stop polyA_site 3039..3052 /label=polya misc_feature 3012..3052 /label=BBa_B0012 terminator 3012..3052 /label=BBa_B0012 misc_feature 2123..3052 /label=BBa_P0451 misc_feature 1213..3052 /label=BBa_K376019 misc_feature 544..3052 /label=BBa_K376025 promoter 3061..3095 /label=BBa_J23116 misc_feature 3104..3902 /label=BBa_K376010 misc_feature 3061..3902 /label=BBa_K376013 misc_feature 544..3902 /label=BBa_K376029 ORIGIN 1 gtattatttg atctttatca accgctaaaa tatgcgtttt gatcttcatc aataaaaaca 61 gcaacttcaa tgtcttaatt actagaggaa attcacacaa atttaaggag agtactagat 121 ggcatcgttc caatccttcg gcatcccagg tcaattagaa gtaatcaaaa aggctctgga 181 ccacgtccgc gtgggcgtcg tgatcaccga tccggcgccg gaggacaacc cgatcgttta 241 cgtgaatcaa ggtttcgttc agatgacggg ttacgaaacc gaagagatcc tgggcaaaaa 301 tgcgcgtttt ctccagggta agcatacgga cccggcggag gttgataaca tccgtaccgc 361 cttgcagaat aaagagccgg ttaccgtgca aattcagaac tataagaaag acggtaccat 421 gttttggaac gagctgaaca ttgacccgat ggaaattgag gacaagactt actttgtcgg 481 cattcagaat gatattacga agcagaaaga atatgagaaa ctgctggaat gataatacta 541 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 601 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 661 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 721 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 781 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 841 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 901 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 961 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 1021 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 1081 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 1141 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 1201 tctctactag agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt 1261 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc 1321 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct 1381 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta 1441 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta 1501 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat 1561 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca 1621 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag 1681 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta 1741 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt 1801 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac 1861 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta 1921 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 1981 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2041 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata 2101 gagatactga gcactactag agaaagagga gaaatactag atgagcacaa aaaagaaacc 2161 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa 2221 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg 2281 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc 2341 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga 2401 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt 2461 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc 2521 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga 2581 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat 2641 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg 2701 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact 2761 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat 2821 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt 2881 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa 2941 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3001 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 3061 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 3121 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 3181 aagcaatatt gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 3241 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 3301 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 3361 tatagtaggt tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 3421 tgctgtaaat aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact 3481 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat 3541 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta 3601 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac 3661 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct 3721 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat 3781 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca 3841 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca 3901 ct //