virG

BBa_K389002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K389002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Aretz
Date created: 2010-08-09 11:00:00
Date modified: 2015-05-08 01:12:18

Mutated virG



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K389002_sequence (Version 1)

Description

This is the virG response regulator from the virA/G receptor system. The virA/G receptor system is used by Agrobacterium tumefaciens to detect phenolic substances secreted by plants. In presence of these substances, virG is activated by the virA receptor and induces the transcription of genes under the control of a vir promotor. Normally, virG needs the rpoA RNA polymerase subunit from A. tumefaciens to work in Escherichia coli but this virG BioBrick is mutated, so works with the rpoA subunit from E. coli.

Notes

synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites

Source

synthetic

Sequence Annotation Location Component / Role(s)
RBS
virG
Stop codon
4,8
12,737
738,740
ribosome_entry_site feature/rbs
CDS feature/protein
stop_codon feature/stop
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BBa_K389002/1