Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Aretz
Date created: 2010-08-09 11:00:00
Date modified: 2015-05-08 01:12:19
VirA screening device
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K389011_sequence (Version 1) |
Description
This part contains:- a mutated virG response regulator from the virA/G receptor system from Agrobacterium tumefaciens which works in Escherichia coli without an rpoA gene from A. tumefaciens
- a kanamycin resistance under the control of a vir promotor
This device is used to screen virA receptor mutants. The virA receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more virG is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
Notes
medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance lowSource
synthetic virG gene, vir promotor from A. tumefaciens C58, partsregistry| Access | Instance | Definition |
| public public public public public public | BBa_J23110 BBa_K389002 BBa_B0010 BBa_B0010 BBa_K389003 BBa_K389005 | BBa_J23110 virG BBa_B0010 BBa_B0010 Pvir kanR |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_K389002 BBa_B0010 BBa_B0010 BBa_K389003 BBa_K389005 | 1,35 44,783 792,871 880,959 968,1125 1132,1950 | BBa_J23110 virG BBa_B0010 BBa_B0010 Pvir kanR |