LOCUS       BBa_K389011             1950 bp    DNA     linear       04-May-2024
DEFINITION  VirA screening device
ACCESSION   BBa_K389011
VERSION     BBa_K389011.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             47..51
                     /label=RBS
     CDS             55..780
                     /label=virG
     misc_feature    781..783
                     /label=Stop codon
     CDS             44..783
                     /label=BBa_K389002
     stem_loop       803..846
                     /label=stem_loop
     misc_feature    792..871
                     /label=BBa_B0010
     terminator      792..871
                     /label=BBa_B0010
     stem_loop       891..934
                     /label=stem_loop
     misc_feature    880..959
                     /label=BBa_B0010
     terminator      880..959
                     /label=BBa_B0010
     misc_feature    1000..1011
                     /label=vir-box
     RBS             1120..1124
                     /label=rbs
     promoter        1056..1060
                     /label=-35
     promoter        1080..1084
                     /label=-10
     promoter        968..1125
                     /label=BBa_K389003
     CDS             1132..1947
                     /label=kanamycin resistance
     misc_feature    1948..1950
                     /label=Stop codon
     CDS             1132..1950
                     /label=BBa_K389005
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaagagga gaaaatgaaa
       61 cacgtcctgc tggttgatga tgatgtggct atgcgtcacc tgattatcga gtatctgacc
      121 atccatgcct tcaaagtcac agccgttgcc gattccactc agtttacccg tgtactgtct
      181 agtgctaccg tcgatgttgt cgtggtcgat ctgaatctgg ttcgtgaaga tggcctggaa
      241 attgtccgta acctggcagc aaaaagcgat atccctatca ttgtgatcag cggggatcgt
      301 ctggaggaaa cagacaaagt tgttgccctg gagctgggtg cttctgattt tattgccaaa
      361 ccgttcagca tccgtgagtt tctggcccgt attcgtgtag ccctgcgcgt tcgtcctaac
      421 gtcgttcgtt cgaaagaccg tcgttccttt tgttttaccg actggaccct gaatctgcgt
      481 caacgtcgcc tgatgagcga agctggcggt gaagtgaaac tgacagccgg tgagttcaat
      541 ctgctgctgg catttctgga aaaacctcgt gatgttctga gccgtgaaca gctgctgatt
      601 gcctctcgtg tccgtgatga agaggtgtat gatcgttcta tcgatgtcct gattctgcgc
      661 ctgcgtcgca aactggaagc cgatccgtct agtcctcagc tgattaaaac cgcccgtggt
      721 gctgggtatt tcttcgatgc cgatgtccaa gtgtcacatg gtggcacgat ggctgcttaa
      781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagc caggcatcaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      961 actagaggca cccttaagtg catggaaagc cgttttcgct tcaaatgaaa tcgaaaagaa
     1021 gaaaacaaaa atcctagagt aaccgaccct cccgataatc gtgaacatca gatgacagca
     1081 tttcttccga ccgaagtggc tgtgttggtt atgagcttga ggagatacta gatgagccat
     1141 attcaacggg aaacgtcttg ctcccgtccg cgcttaaact ccaacatgga cgctgattta
     1201 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1261 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1321 gatgttacag atgagatggt ccgtctcaac tggctgacgg agtttatgcc tctcccgacc
     1381 atcaagcatt ttatccgtac tcctgatgat gcgtggttac tcaccaccgc gattcctggg
     1441 aaaacagcct tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1501 ctggccgtgt tcctgcgccg gttacattcg attcctgttt gtaattgtcc ttttaacagc
     1561 gatcgtgtat ttcgtcttgc tcaggcgcaa tcacgcatga ataacggttt ggttgatgcg
     1621 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcac
     1681 aagctcttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1741 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgggt cggaatcgca
     1801 gaccgttacc aggaccttgc cattctttgg aactgcctcg gtgagttttc tccttcatta
     1861 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1921 catttgatgc tcgatgagtt tttctaataa
//