LOCUS       BBa_K390000             2157 bp    DNA     linear       12-Jun-2024
DEFINITION  pSB1A3_Int (BamHI, XhoI, NdeI, NheI added)
ACCESSION   BBa_K390000
VERSION     BBa_K390000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=suffix
     misc_feature    22..93
                     /label=E. coli his operon terminator
     stem_loop       30..64
                     /label=terminator stem loop
     misc_feature    34..35
                     /label=deletion of two bases
     primer_bind     157..176
                     /label=VR
     misc_feature    216..221
                     /label=NdeI site
     misc_feature    219..219
                     /label=g > a
     misc_feature    230..235
                     /label=NheI Site
     misc_feature    232..232
                     /label=c > t
     CDS             261..875
                     /label=rep(pMB1) (rev)
     misc_feature    276..276
                     /label=Ori
     CDS             1025..1895
                     /label=AmpR (rev)
     misc_feature    1992..1997
                     /label=BamHI Site
     misc_feature    1995..1995
                     /label=t > a
     misc_feature    2009..2014
                     /label=XhoI Site
     misc_feature    2010..2010
                     /label=c > t
     misc_feature    2014..2014
                     /label=a > g
     primer_bind     2017..2037
                     /label=VF2
     misc_feature    2094..2097
                     /label=end of terminator
     stem_loop       2107..2126
                     /label=terminator stem loop
     misc_feature    2136..2157
                     /label=prefix
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      181 ttatccacag aatcagggga taacgcagga aagaacatat gagcaaaagg ctagcaaaag
      241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggatccgcg cacatttcct cgagaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     2101 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag
//