Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Tramp
Date created: 2010-07-31 11:00:00
Date modified: 2015-05-08 01:12:19
5'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 6803
| Types | DnaRegion |
| Roles | RBS ribosome_entry_site |
| Sequences | BBa_K390002_sequence (Version 1) |
Description
This is the 5'UTR and RBS region from gene psbA2 in Synechocystis sp. PCC 6803. It covers the entire region from the end of the promoter (as reported in Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3: 65-87) to immediately before the start codon of the psbA2 gene.The RBS region has been mutated from the native RBS "taaggaatta" to the consensus RBS "aaaggaggtt" for Synechocystis sp. PCC 6803.
This part can serve as a general RBS for Synechocystis sp. PCC 6803, until a library of RBS strengths in PCC 6803 is assembled. The consensus RBS was determined by aligning the 9 nucleotides on the 3' end sequence of the 16S rRNA to the 20 nucleotides preceding the start codon of every gene in PCC 6803. The most frequently represented nucleotide at each location along the alignment was considered to be the consensus. (Note: the alignment was done in relation to the 16S rRNA sequence, and not the start codon, as the spacing between RBS and the start codon varied considerably from gene to gene).
Notes
noneSource
Based on genomic sequence in Synechocystis sp. PCC 6803. Made from annealing of two oligionucleotides that contained the mutations to the consensus RBS.| Sequence Annotation | Location | Component / Role(s) |
| 5' UTR with consensus RBS consensus RBS SDS core consensus t > a at > gg a > t | 1,49 33,42 35,40 33,33 39,40 42,42 | feature/misc sequence_feature ribosome_entry_site feature/rbs ribosome_entry_site feature/rbs sequence_alteration feature/mutation sequence_alteration feature/mutation feature/mutation sequence_alteration |