LOCUS       BBa_K390034              957 bp    DNA     linear       01-Jun-2024
DEFINITION  sigF promoter, 5' UTR and native RBS, and GFP
ACCESSION   BBa_K390034
VERSION     BBa_K390034.1  
FEATURES             Location/Qualifiers
     promoter        1..37
                     /label=sigF promoter
     misc_feature    6..10
                     /label=-32 box
     misc_feature    25..29
                     /label=-10 box
     promoter        1..37
                     /label=BBa_K390009
     misc_feature    46..94
                     /label=5' UTR with native RBS
     RBS             80..83
                     /label=SDS core consensus
     RBS             78..87
                     /label=native RBS
     RBS             46..94
                     /label=BBa_K390001
     misc_feature    101..103
                     /label=Start
     CDS             101..820
                     /label=GFP Coding Region
     CDS             101..820
                     /label=BBa_K208000
     stem_loop       840..883
                     /label=stem_loop
     misc_feature    829..908
                     /label=BBa_B0010
     terminator      829..908
                     /label=BBa_B0010
     stem_loop       924..943
                     /label=T7 TE
     misc_feature    950..950
                     /label=stop
     polyA_site      944..957
                     /label=polya
     misc_feature    917..957
                     /label=BBa_B0012
     terminator      917..957
                     /label=BBa_B0012
     misc_feature    101..957
                     /label=BBa_K208014
ORIGIN
        1 tgatgtaggc agagtccaac catcggtaaa gttgatttac tagagagtca gttccaatct
       61 gaacatcgac aaatacataa ggaattataa ccaatactag atggctagca aaggagaaga
      121 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      181 attttctgtc agtggagagg gtgaaggtga tgctacatac ggaaagctta cccttaaatt
      241 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
      301 tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact ttttcaagag
      361 tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaacta
      421 caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa
      481 aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt acaactataa
      541 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
      601 aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc aacaaaatac
      661 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgc
      721 cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc
      781 tgctgggatt acacatggca tggatgagct ctacaaataa tactagagcc aggcatcaaa
      841 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      901 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//