LOCUS       BBa_K390040              966 bp    DNA     linear       01-Jun-2024
DEFINITION  sigE promoter, 5' UTR and consensus RBS, and GFP
ACCESSION   BBa_K390040
VERSION     BBa_K390040.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=sigE promoter
     misc_feature    1..14
                     /label=NctA enhancer binding site
     misc_feature    36..41
                     /label=-10 box
     promoter        1..46
                     /label=BBa_K390007
     misc_feature    55..103
                     /label=5' UTR with consensus RBS
     RBS             87..96
                     /label=consensus RBS
     RBS             89..94
                     /label=SDS core consensus
     misc_feature    87..87
                     /label=t > a
     misc_feature    93..94
                     /label=at > gg
     misc_feature    96..96
                     /label=a > t
     RBS             55..103
                     /label=BBa_K390002
     misc_feature    110..112
                     /label=Start
     CDS             110..829
                     /label=GFP Coding Region
     CDS             110..829
                     /label=BBa_K208000
     stem_loop       849..892
                     /label=stem_loop
     misc_feature    838..917
                     /label=BBa_B0010
     terminator      838..917
                     /label=BBa_B0010
     stem_loop       933..952
                     /label=T7 TE
     misc_feature    959..959
                     /label=stop
     polyA_site      953..966
                     /label=polya
     misc_feature    926..966
                     /label=BBa_B0012
     terminator      926..966
                     /label=BBa_B0012
     misc_feature    110..966
                     /label=BBa_K208014
ORIGIN
        1 gtatcacgaa ttacactgcc gtgaaaattt aacgatattt tggacatact agagagtcag
       61 ttccaatctg aacatcgaca aatacaaaag gaggtttaac caatactaga tggctagcaa
      121 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      181 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gctacatacg gaaagcttac
      241 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      301 tttctcttat ggtgttcaat gcttttcccg ttatccggat catatgaaac ggcatgactt
      361 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga
      421 cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat
      481 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca aactcgagta
      541 caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc
      601 taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag accattatca
      661 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac
      721 acaatctgcc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt
      781 tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaataat actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttata
//