LOCUS       BBa_K390047              948 bp    DNA     linear       12-Jun-2024
DEFINITION  hspA promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390047
VERSION     BBa_K390047.1  
FEATURES             Location/Qualifiers
     promoter        4..41
                     /label=hspA promoter
     RBS             73..79
                     /label=putative rbs
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     misc_feature    42..42
                     /label=+1 Transcription Start Point
     promoter        1..85
                     /label=BBa_K390014
     misc_feature    92..94
                     /label=Start
     CDS             92..811
                     /label=GFP Coding Region
     CDS             92..811
                     /label=BBa_K208000
     stem_loop       831..874
                     /label=stem_loop
     misc_feature    820..899
                     /label=BBa_B0010
     terminator      820..899
                     /label=BBa_B0010
     stem_loop       915..934
                     /label=T7 TE
     misc_feature    941..941
                     /label=stop
     polyA_site      935..948
                     /label=polya
     misc_feature    908..948
                     /label=BBa_B0012
     terminator      908..948
                     /label=BBa_B0012
     misc_feature    92..948
                     /label=BBa_K208014
ORIGIN
        1 aaccgaatct aacctggaag gggaaatttt aagatagaac cattcaaggg taatcaattc
       61 cttccacaca tcaggagtta acatttacta gatggctagc aaaggagaag aacttttcac
      121 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      181 cagtggagag ggtgaaggtg atgctacata cggaaagctt acccttaaat ttatttgcac
      241 tactggaaaa ctacctgttc catggccaac acttgtcact actttctctt atggtgttca
      301 atgcttttcc cgttatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc
      361 cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg
      421 tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga
      481 ttttaaagaa gatggaaaca ttctcggaca caaactcgag tacaactata actcacacaa
      541 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
      601 caacattgaa gatggatccg ttcaactagc agaccattat caacaaaata ctccaattgg
      661 cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg ccctttcgaa
      721 agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat
      781 tacacatggc atggatgagc tctacaaata atactagagc caggcatcaa ataaaacgaa
      841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//