LOCUS       BBa_K390050              950 bp    DNA     linear       12-Jun-2024
DEFINITION  psbA2 promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390050
VERSION     BBa_K390050.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=psbA2 promoter
     RBS             71..80
                     /label=putative rbs
     misc_feature    3..8
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    39..39
                     /label=+1 Transcription Start Point
     promoter        1..87
                     /label=BBa_K390017
     misc_feature    94..96
                     /label=Start
     CDS             94..813
                     /label=GFP Coding Region
     CDS             94..813
                     /label=BBa_K208000
     stem_loop       833..876
                     /label=stem_loop
     misc_feature    822..901
                     /label=BBa_B0010
     terminator      822..901
                     /label=BBa_B0010
     stem_loop       917..936
                     /label=T7 TE
     misc_feature    943..943
                     /label=stop
     polyA_site      937..950
                     /label=polya
     misc_feature    910..950
                     /label=BBa_B0012
     terminator      910..950
                     /label=BBa_B0012
     misc_feature    94..950
                     /label=BBa_K208014
ORIGIN
        1 gctttacaaa actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc
       61 gacaaataca taaggaatta taaccaatac tagatggcta gcaaaggaga agaacttttc
      121 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      181 gtcagtggag agggtgaagg tgatgctaca tacggaaagc ttacccttaa atttatttgc
      241 actactggaa aactacctgt tccatggcca acacttgtca ctactttctc ttatggtgtt
      301 caatgctttt cccgttatcc ggatcatatg aaacggcatg actttttcaa gagtgccatg
      361 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggaa ctacaagacg
      421 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt
      481 gattttaaag aagatggaaa cattctcgga cacaaactcg agtacaacta taactcacac
      541 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
      601 cacaacattg aagatggatc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      661 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgccctttcg
      721 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
      781 attacacatg gcatggatga gctctacaaa taatactaga gccaggcatc aaataaaacg
      841 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      901 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//