LOCUS       BBa_K390056             1173 bp    DNA     linear       12-Jun-2024
DEFINITION  sigE promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390056
VERSION     BBa_K390056.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=sigE promoter
     misc_feature    36..41
                     /label=-10 box
     misc_feature    1..14
                     /label=NtcA binding site
     promoter        1..310
                     /label=BBa_K390023
     misc_feature    317..319
                     /label=Start
     CDS             317..1036
                     /label=GFP Coding Region
     CDS             317..1036
                     /label=BBa_K208000
     stem_loop       1056..1099
                     /label=stem_loop
     misc_feature    1045..1124
                     /label=BBa_B0010
     terminator      1045..1124
                     /label=BBa_B0010
     stem_loop       1140..1159
                     /label=T7 TE
     misc_feature    1166..1166
                     /label=stop
     polyA_site      1160..1173
                     /label=polya
     misc_feature    1133..1173
                     /label=BBa_B0012
     terminator      1133..1173
                     /label=BBa_B0012
     misc_feature    317..1173
                     /label=BBa_K208014
ORIGIN
        1 gtatcacgaa ttacactgcc gtgaaaattt aacgatattt tggacagata gatattaaaa
       61 aaaactaaag ttttgccatt tttggaaaat caatgacaca atctagccac ggggactggt
      121 atctggttct ggaacaccac taccgcagtg cgcatagctg aaagtggaga gccttaaccg
      181 ttggggtggt ctatctggat gatgctctgg ccgattggat aatttctggt tgttttctgt
      241 ttccttgggt ggccttgttg ttgccacatc ttttcctttc tcgattgttt ccgtttagtt
      301 ttttcaacct tactagatgg ctagcaaagg agaagaactt ttcactggag ttgtcccaat
      361 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      421 aggtgatgct acatacggaa agcttaccct taaatttatt tgcactactg gaaaactacc
      481 tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcccgtta
      541 tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      601 ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg aagtcaagtt
      661 tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta aagaagatgg
      721 aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat acatcacggc
      781 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg
      841 atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      901 tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa
      961 gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac atggcatgga
     1021 tgagctctac aaataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1081 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1141 gctcaccttc gggtgggcct ttctgcgttt ata
//