LOCUS       BBa_K392038             2628 bp    DNA     linear       17-May-2024
DEFINITION  GFP surface display testing construct for yeast
ACCESSION   BBa_K392038
VERSION     BBa_K392038.1  
FEATURES             Location/Qualifiers
     promoter        1..1445
                     /label=Constitutive promoter
     misc_feature    1348..1354
                     /label=TATA Box
     promoter        1..1445
                     /label=BBa_J63005
     CDS             1452..1712
                     /label=Aga2 CDS
     misc_feature    1452..1712
                     /label=BBa_K416000
     misc_feature    1721..1765
                     /label=BBa_K416001
     misc_feature    1452..1765
                     /label=BBa_K416004
     CDS             1774..2491
                     /label=GFP fusion brick
     misc_feature    1774..1774
                     /label=deletion of TG
     CDS             1774..2491
                     /label=BBa_K125500
     misc_feature    2516..2522
                     /label=TATA box
     promoter        2500..2602
                     /label=BBa_K105027
     misc_feature    2623..2625
                     /label=start codon
     RBS             2611..2628
                     /label=BBa_J63003
     terminator      2500..2628
                     /label=BBa_K392003
ORIGIN
        1 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa
       61 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg
      121 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg
      181 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat
      241 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg
      301 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct
      361 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt
      421 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct
      481 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat
      541 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt
      601 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag
      661 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta
      721 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc
      781 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
      841 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
      901 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
      961 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     1021 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     1081 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa atgatggaag acactaaagg
     1141 aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt
     1201 atcttcgaac acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc
     1261 aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaagt ttgccgcttt
     1321 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     1381 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     1441 caacttacta gatgcagtta cttcgctgtt tttcaatatt ttctgttatt gcttcagttt
     1501 tagcacagga actgacaact atatgcgagc aaatcccctc accaacttta gaatcgacgc
     1561 cgtactcttt gtcaacgact actattttgg ccaacgggaa ggcaatgcaa ggagtttttg
     1621 aatattacaa atcagtaacg tttgtcagta attgcggttc tcacccctca acaactagca
     1681 aaggcagccc cataaacaca cagtatgttt tttactagag ggtggaggag gctctggtgg
     1741 aggcggtagc ggaggcggag ggtcgtacta gagcgtaaag gagaagaact tttcactgga
     1801 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     1861 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     1921 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     1981 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     2041 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     2101 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     2161 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     2221 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     2281 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2341 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     2401 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2461 catggcatgg atgaactata caaataataa ttactagagg catgtgctct gtatgtatat
     2521 aaaactcttg ttttcttctt ttctctaaat attctttcct tatacattag gacctttgca
     2581 gcataaatta ctatacttct attactagag cccgccgcca ccatggag
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