BBa_I742158 1 rbs+crtZ crtZ (beta-carotene hydroxylase) with native rbs 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Released HQ 2013 crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin. false false _123_ 0 837 163 In stock true This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence. true Chris French component1953815 1 BBa_I742157 component1953813 1 BBa_I742156 annotation1953815 1 BBa_I742157 range1953815 1 17 547 annotation1953813 1 BBa_I742156 range1953813 1 1 16 BBa_K118012 1 BBa_K118012 Synthetic ribosome binding site added by Son of Babel procedure 2008-08-18T11:00:00Z 2015-05-08T01:09:36Z Synthetic DNA This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system. false false _192_ 0 3282 9 Not in stock false The part can be added by fusion PCR using a Son of Babel reverse primer. false Andrew Hall BBa_K118003 1 BBa_K118003 crtI coding sequence encoding phytoene dehydrogenase 2008-07-23T11:00:00Z 2015-05-08T01:09:36Z Pantoea ananatis genomic DNA Released HQ 2013 This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene. false false _192_ 0 3282 9 In stock true The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation. true Andrew Hall annotation1995702 1 crtI coding sequence range1995702 1 1 1479 annotation1968245 1 PstI site removed range1968245 1 889 894 annotation1968244 1 PstI site removed range1968244 1 478 483 BBa_I732100 1 LacI LacI 2007-10-13T11:00:00Z 2016-10-18T06:58:21Z N/A Released HQ 2013 N/A false false _156_ 31248 1557 9 In stock false N/A true Zhan Jian BBa_I15009 1 BBa_I15009 phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis 2004-09-19T11:00:00Z 2015-08-31T04:07:38Z Voigt Lab via Anselm Levskaya Released HQ 2013 Second of two required phycocyanobilin biosynthetic genes. false false _5_ 0 88 7 In stock true true Jeff Tabor annotation1891612 1 PcyA range1891612 1 1 750 annotation2214032 1 Help:Barcodes range2214032 1 751 775 BBa_K395705 1 BBa_K395705 crtW (beta-carotene ketolase) with rbs 2010-10-05T11:00:00Z 2015-05-08T01:12:22Z Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin. false false _505_ 0 6372 9 It's complicated true No special considerations. Please refer to Part BBa_K395706 for more information. false Yumiko Kinoshita component2082981 1 BBa_K395700 component2082980 1 BBa_J15001 annotation2082981 1 BBa_K395700 range2082981 1 17 751 annotation2082980 1 BBa_J15001 range2082980 1 1 10 BBa_C0051 1 cI lam cI repressor from E. coli phage lambda (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Released HQ 2013 Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P> true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2213991 1 Help:Barcodes range2213991 1 751 775 annotation23334 1 cI lambda range23334 1 4 711 annotation23335 1 LVA range23335 1 712 744 BBa_I742157 1 BBa_I742157 crtZ (beta-carotene hydroxylase) coding sequence 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Coding sequence for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxanthin. false true _123_ 0 837 163 Not in stock false No special considerations. false Chris French annotation1953812 1 crtZ range1953812 1 1 528 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_R1051 1 cI lam Promoter, Standard (lambda cI regulated)<br> 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].<br><br>The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false false _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<br><br> Modified to comply with BBa_S0001:<br> TTGACA-17N-GATACT<br><P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross<br> Drew Endy<br> annotation2078 1 -10 range2078 1 38 43 annotation2076 1 OR1 range2076 1 25 41 annotation2075 1 -35 range2075 1 15 20 annotation2077 1 OR2 range2077 1 1 17 annotation7074 1 BBa_R1051 range7074 1 1 49 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_I0462 1 LuxR luxR Protein Generator 2003-12-04T12:00:00Z 2015-08-31T04:07:29Z Released HQ 2013 Produces LuxR protein which can sense 3OC₆HSL in the media and activate transcription from R0062, the right hand Lux promoter false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff component943181 1 BBa_C0062 component943199 1 BBa_B0012 component943189 1 BBa_B0010 component943166 1 BBa_B0034 annotation943181 1 BBa_C0062 range943181 1 19 774 annotation943166 1 BBa_B0034 range943166 1 1 12 annotation943199 1 BBa_B0012 range943199 1 896 936 annotation943189 1 BBa_B0010 range943189 1 808 887 BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2045 1 LuxR/HSL range2045 1 1 20 annotation2046 1 -35 range2046 1 20 25 annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2047 1 -10 range2047 1 42 47 annotation2048 1 start range2048 1 53 53 BBa_K395407 1 BBa_K395407 pompC(CS1)-lacIM1 2010-10-04T11:00:00Z 2015-05-08T01:12:21Z test lacIM1 regulated by ompC-CS1 promoter.The promoter is regulated by phosphorylated OmpR. false false _505_ 0 6396 9 Not in stock false test false Mitsuhiko Odera component2082494 1 BBa_K082005 component2082495 1 BBa_B0010 component2082497 1 BBa_B0012 component2082491 1 BBa_K395303 component2082493 1 BBa_B0034 annotation2082493 1 BBa_B0034 range2082493 1 68 79 annotation2082497 1 BBa_B0012 range2082497 1 1265 1305 annotation2082495 1 BBa_B0010 range2082495 1 1177 1256 annotation2082494 1 BBa_K082005 range2082494 1 86 1168 annotation2082491 1 BBa_K395303 range2082491 1 1 59 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K274200 1 BBa_K274200 CrtEBIY with rbs 2009-09-17T11:00:00Z 2015-05-08T01:11:45Z Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006, Part BBa_K118005 and Part BBa_K118013. This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs), Part BBa_K118005 (CrtI with rbs) and Part BBa_K118013 (CrtY with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006, BBa_K118005 and BBa_K118013 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274210) and arabinose-inducible promoter I0500 (see Part BBa_274220), transformed and tested in E.coli strain MG1655. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). A related Composite Biobrick is Part BBa_K274100 (CrtEBI with rbs), which "stops" at one step before beta-carotene, producing (and retaining) red lycopene pigment. false true _374_ 0 5416 9 In stock false The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (beta-carotene) production than strain TOP10. false Siming Ma component2021596 1 BBa_K118008 component2021593 1 BBa_K118003 component2021584 1 BBa_J15001 component2021586 1 BBa_K118002 component2021581 1 BBa_I742151 component2021580 1 BBa_K118012 component2021589 1 BBa_J15001 component2021594 1 BBa_K118012 annotation2021596 1 BBa_K118008 range2021596 1 3404 4555 annotation2021580 1 BBa_K118012 range2021580 1 1 4 annotation2021581 1 BBa_I742151 range2021581 1 11 922 annotation2021589 1 BBa_J15001 range2021589 1 1888 1897 annotation2021593 1 BBa_K118003 range2021593 1 1904 3385 annotation2021594 1 BBa_K118012 range2021594 1 3394 3397 annotation2021586 1 BBa_K118002 range2021586 1 947 1879 annotation2021584 1 BBa_J15001 range2021584 1 931 940 BBa_C0078 1 lasI autoinducer synthetase for PAI from Pseudomonas aeruginosa 2004-01-27T12:00:00Z 2015-08-31T04:07:24Z www.ncbi.nlm.nih.gov Released HQ 2013 coding region for lasI protein, which produces the chemical signal AI-1 false false _1_ 0 24 7 In stock false true Chris Walsh (Fighting Darwins) annotation305970 1 LasI range305970 1 1 603 annotation2214003 1 Help:Barcodes range2214003 1 643 667 annotation306608 1 LVA range306608 1 604 636 BBa_I15008 1 BBa_I15008 heme oxygenase (ho1) from Synechocystis 2004-09-19T11:00:00Z 2015-08-31T04:07:38Z Voigt Lab via Anselm Levskaya Released HQ 2013 One of two requisite genes required for the biosynthesis of phycocyanobilin from heme. false false _5_ 0 88 7 In stock true true Jeff Tabor annotation2214026 1 Help:Barcodes range2214026 1 727 751 BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301166 1 -35 range301166 1 75 80 annotation301156 1 C3 OmpR range301156 1 54 71 annotation301167 1 -10 range301167 1 98 103 annotation301155 1 C2 OmpR range301155 1 34 51 annotation301154 1 C1 OmpR range301154 1 13 30 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 BBa_K118002 1 BBa_K118002 crtB coding sequence encoding phytoene synthase 2008-07-23T11:00:00Z 2015-05-08T01:09:36Z Pantoea ananatis genomic DNA Released HQ 2013 This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990) false false _192_ 0 3282 9 In stock true No special considerations. true Andrew Hall annotation1995701 1 crtB coding sequence range1995701 1 1 930 BBa_J23107 1 BBa_J23107 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false false _52_ 0 483 95 It's complicated true N/A true John Anderson BBa_I742151 1 BBa_I742151 crtE (geranylgeranyl pyrophosphate synthase) coding sequence. 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway. false false _123_ 0 837 163 Not in stock false No important considerations. false Chris French BBa_I742156 1 BBa_I742156 crtZ native rbs 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. false false _123_ 0 837 163 Not in stock false A virtual part to be added to the coding sequence blunt (without a scar). false Chris French BBa_K081016 1 BBa_K081016 lasI protein generator - PoPS->lasI 2008-10-18T11:00:00Z 2015-05-08T01:08:35Z RBS: lasI: T: Released HQ 2013 PoPS in -> lasI out. <br> Strong RBS (efficiency=0.6). <br> Artificial 39 bp terminator (efficiency=0.99). false false _227_ 0 2583 9 In stock true We used BioBrick Standard Assembly. true Lorenzo Pasotti, Paolo Magni component2246162 1 BBa_C0078 component2246167 1 BBa_B1006 component2246157 1 BBa_B0030 annotation2246157 1 BBa_B0030 range2246157 1 1 15 annotation2246162 1 BBa_C0078 range2246162 1 22 688 annotation2246167 1 BBa_B1006 range2246167 1 697 735 BBa_K395162 1 BBa_K395162 CmR activated by LuxR and 3OC6HSL(AHL of Lux family) 2010-10-04T11:00:00Z 2015-05-08T01:12:21Z this part express chloramphenicol resistance by the activation of LuxR and 3OC6HSL false false _505_ 0 6371 9 In stock true We want to create chloramphenicol resistance gene on pSB6A1.As a vector,We already create chloramphenicol resistance gene on pSB6A1and promotor exist as biobrick(BBa_R0062).To accomplish it, vector was cutted by EcoRI and Xha while insert(promotor) was cutted by EcoRI and SpeI false Kaneta Yusuke component2104722 1 BBa_R0062 component2104728 1 BBa_K395160 annotation2104722 1 BBa_R0062 range2104722 1 1 55 annotation2104728 1 BBa_K395160 range2104728 1 64 742 BBa_K118008 1 BBa_K118008 crtY coding sequence encoding lycopene B-cyclase 2008-08-18T11:00:00Z 2015-05-08T01:09:36Z Pantoea ananatis genomic DNA Released HQ 2013 This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene. false false _192_ 0 3282 9 In stock true No special considerations true Andrew Hall annotation1995716 1 crtY coding sequence range1995716 1 1 1149 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K395604 1 BBa_K395604 MpAAT1 coding region 2010-10-25T11:00:00Z 2015-05-08T01:12:22Z ATGA TGTCCTTTTC CGTACTGCAA GTGAAACGTC TGCAACCGGA 301 ACTGATTACA CCGGCAAAAT CAACCCCTCA GGAAACCAAA TTTCTGAGCG ACATCGATGA 361 TCAAGAATCT CTGCGCGTTC AGATTCCGAT TATCATGTGT TATAAAGACA ACCCGAGCCT 421 GAATAAAAAC CGTAACCCGG TCAAAGCCAT TCGTGAAGCA CTGTCACGTG CCCTGGTCTA 481 TTATTATCCA CTGGCCGGTC GTCTGCGTGA AGGTCCAAAC CGTAAACTGG TGGTCGATTG 541 TAACGGCGAA GGCATCCTGT TTGTGGAAGC TTCCGCCGAT GTTACGCTGG AACAGCTGGG 601 GGACAAAATC CTGCCTCCTT GTCCTCTGCT GGAGGAGTTT CTGTATAACT TCCCGGGTTC 661 TGATGGCATT ATCGACTGCC CACTGCTGCT GATTCAAGTG ACCTGTCTGA CTTGTGGAGG 721 GTTCATCCTG GCTCTGCGCC TGAACCATAC AATGTGTGAT GCCGCCGGTC TGCTGCTGTT 781 TCTGACCGCT ATTGCCGAAA TGGCCCGTGG CGCTCATGCC CCTAGTATTC TGCCTGTCTG 841 GGAGCGTGAA CTGCTGTTTG CTCGTGATCC TCCTCGTATC ACCTGTGCCC ACCATGAATA 901 TGAGGACGTG ATTGGCCACT CAGATGGGTC TTATGCCAGC TCAAATCAGA GTAATATGGT 961 CCAACGCTCG TTCTATTTTG GCGCCAAAGA GATGCGCGTA CTGCGTAAAC AAATTCCGCC 1021 ACACCTGATT AGCACTTGTA GCACCTTCGA TCTGATCACC GCTTGTCTGT GGAAATGTCG 1081 TACCCTGGCA CTGAACATTA ACCCGAAAGA GGCCGTTCGT GTTTCGTGTA TTGTGAATGC 1141 TCGTGGTAAA CACAACAACG TTCGCCTGCC TCTGGGTTAT TATGGTAACG CCTTTGCCTT 1201 TCCGGCAGCA ATTTCAAAAG CCGAACCTCT GTGTAAAAAC CCGCTGGGAT ATGCTCTGGA 1261 ACTGGTAAAA AAAGCGAAAG CGACTATGAA CGAAGAGTAT CTGCGTTCTG TTGCTGATCT 1321 GCTGGTACTG CGCGGTCGCC CTCAGTATAG TAGTACCGGC TCCTATCTGA TCGTTAGCGA 1381 CAACACCCGT GTAGGCTTCG GAGATGTGAA TTTTGGCTGG GGTCAGCCTG TTTTTGCTGG 1441 ACCGGTTAAA GCACTGGATC TGATCTCGTT TTATGTCCAA CACAAAAACA ACACCGAGGA 1501 TGGCATTCTG GTTCCGATGT GTCTGCCTAG TAGCGCTATG GAACGCTTTC AGCAGGAACT 1561 GGAGCGTATT ACCCAGGAAC CAAAAGAGGA CATCTGTAAC AATCTGCGCT CAACGTCCCA 1621 GTAA MpAAT1 coding region false false _505_ 0 7030 9 Not in stock false a false Toshitaka Matsubara annotation2106200 1 start range2106200 1 24 24 annotation2106205 1 stop range2106205 1 1532 1532 annotation2106202 1 s-tag range2106202 1 78 111 annotation2106219 1 His-tag range2106219 1 1611 1628 annotation2106203 1 factor-Xa range2106203 1 153 164 annotation2106204 1 MpAAT1 range2106204 1 165 1532 annotation2106199 1 rbs range2106199 1 10 14 annotation2106201 1 his-tag range2106201 1 27 44 BBa_J15001 1 BBa_J15001 strong synthetic E. coli ribosome binding site with SacI site. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Synthetic. This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector. false false _163_ 0 837 163 Not in stock false Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest. false Chris French annotation1938045 1 SacI range1938045 1 1 3 annotation1938046 1 rbs range1938046 1 4 10 BBa_I15010 1 BBa_I15010 cph8 (Cph1/EnvZ fusion) 2004-09-19T11:00:00Z 2015-08-31T04:07:38Z Voigt Lab via Anselm Levskaya Chimeric Cph1 light receptor/EnvZ protein. false false _5_ 0 88 7 In stock true Silent mutation at base 108 (G-A) to remove PstI site false Jeff Tabor annotation2214031 1 Help:Barcodes range2214031 1 2239 2263 annotation1891613 1 Cph8 range1891613 1 1 2238 BBa_K395603 1 BBa_K395603 alcohol acetyltransferase; converts butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl ace 2010-10-18T11:00:00Z 2015-05-08T01:12:22Z Apple BBa_K395603 encodes alcohol acetyltransferase derived from MpAAT1 from Malus pumila cv. Royal Gala. MpAAT1 catalyzes the conversion of butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl acetate. Butyl acetate and 2-methylbutyl acetate have a apple flavor. false false _505_ 0 7030 9 Not in stock false RBS+lac operator+RBS+His tag+thirombin+S&#12539;tag+Factor Xa+MpAAT1+His tag+T7 terminator false Toshitaka Matsubara component2106229 1 BBa_K395601 component2106228 1 BBa_K395604 annotation2106228 1 BBa_K395604 range2106228 1 1 1687 annotation2106229 1 BBa_K395601 range2106229 1 1696 1743 BBa_K395601 1 BBa_K395601 T7 terminator 2010-10-01T11:00:00Z 2015-05-08T01:12:21Z CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG Released HQ 2013 T7 terminator false false _505_ 0 7030 9 In stock false terminator of T7 promoter false Toshitaka Matsubara BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_Q04400 1 tetR QPI (B0034.C0040.B0015.R0040) 2003-12-02T12:00:00Z 2015-05-08T01:14:13Z Released HQ 2013 TetR QPI with strong RBS false true _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff component952781 1 BBa_B0034 component952822 1 BBa_R0040 component952791 1 BBa_C0040 component952797 1 BBa_B0010 component952807 1 BBa_B0012 annotation952781 1 BBa_B0034 range952781 1 1 12 annotation952797 1 BBa_B0010 range952797 1 712 791 annotation952791 1 BBa_C0040 range952791 1 19 678 annotation952822 1 BBa_R0040 range952822 1 849 902 annotation952807 1 BBa_B0012 range952807 1 800 840 BBa_R0061 1 luxR Promoter (HSL-mediated luxR repressor) 2004-01-28T12:00:00Z 2015-05-08T01:14:15Z Released HQ 2013 This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site. false true _1_ 0 24 7 In stock false true Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu annotation305863 1 -35 range305863 1 1 6 annotation305877 1 Lux Box range305877 1 6 25 annotation305864 1 -10 range305864 1 25 30 BBa_K082005 1 BBa_K082005 LacI-Mutant 2008-10-27T12:00:00Z 2015-05-08T01:08:35Z None None false false _228_ 0 1560 9 It's complicated false None false Yanting Xue, Binjie Xu annotation1992781 1 BBa_K082005 range1992781 1 1 1083 BBa_K395998 1 BBa_K395998 Wolf coli system 2010-10-18T11:00:00Z 2015-05-08T01:12:22Z test test false false _505_ 0 6396 9 Not in stock false test false Mitsuhiko Odera component2329095 1 BBa_R0082 component2329097 1 BBa_B0034 component2329187 1 BBa_K395162 component2329108 1 BBa_B0015 component2329055 1 BBa_B0034 component2329115 1 BBa_B0034 component2329225 1 BBa_K395603 component2329078 1 BBa_B0015 component2329178 1 BBa_K081016 component2329051 1 BBa_B0034 component2329146 1 BBa_Q04400 component2329165 1 BBa_R0061 component2329214 1 BBa_K395705 component2329209 1 BBa_I742158 component2329101 1 BBa_C0051 component2329226 1 BBa_K395601 component2329058 1 BBa_I15009 component2329068 1 BBa_B0034 component2329089 1 BBa_K395407 component2329071 1 BBa_I15010 component2329123 1 BBa_B0015 component2329116 1 BBa_I732100 component2329161 1 BBa_I0462 component2329205 1 BBa_K274200 component2329053 1 BBa_I15008 component2329065 1 BBa_B0015 component2329066 1 BBa_J23107 component2329049 1 BBa_J23107 component2329110 1 BBa_R1051 component2329124 1 BBa_R0011 annotation2329110 1 BBa_R1051 range2329110 1 6595 6643 annotation2329051 1 BBa_B0034 range2329051 1 44 55 annotation2329053 1 BBa_I15008 range2329053 1 62 812 annotation2329225 1 BBa_K395603 range2329225 1 17226 18968 annotation2329178 1 BBa_K081016 range2329178 1 9856 10590 annotation2329089 1 BBa_K395407 range2329089 1 4228 5532 annotation2329187 1 BBa_K395162 range2329187 1 10599 11340 annotation2329124 1 BBa_R0011 range2329124 1 7901 7954 annotation2329115 1 BBa_B0034 range2329115 1 6652 6663 annotation2329066 1 BBa_J23107 range2329066 1 1759 1793 annotation2329116 1 BBa_I732100 range2329116 1 6670 7755 annotation2329165 1 BBa_R0061 range2329165 1 9818 9847 annotation2329161 1 BBa_I0462 range2329161 1 8874 9809 annotation2329214 1 BBa_K395705 range2329214 1 16467 17217 annotation2329146 1 BBa_Q04400 range2329146 1 7964 8865 annotation2329078 1 BBa_B0015 range2329078 1 4091 4219 annotation2329101 1 BBa_C0051 range2329101 1 5675 6449 annotation2329226 1 BBa_K395601 range2329226 1 18977 19024 annotation2329108 1 BBa_B0015 range2329108 1 6458 6586 annotation2329123 1 BBa_B0015 range2329123 1 7764 7892 annotation2329065 1 BBa_B0015 range2329065 1 1622 1750 annotation2329055 1 BBa_B0034 range2329055 1 821 832 annotation2329058 1 BBa_I15009 range2329058 1 839 1613 annotation2329205 1 BBa_K274200 range2329205 1 11349 15903 annotation2329097 1 BBa_B0034 range2329097 1 5657 5668 annotation2329095 1 BBa_R0082 range2329095 1 5541 5648 annotation2329209 1 BBa_I742158 range2329209 1 15912 16458 annotation2329049 1 BBa_J23107 range2329049 1 1 35 annotation2329068 1 BBa_B0034 range2329068 1 1802 1813 annotation2329071 1 BBa_I15010 range2329071 1 1820 4082 BBa_K395160 1 BBa_K395160 RBS-CmR(no promoter and terminator) 2010-10-01T11:00:00Z 2015-05-08T01:12:21Z aaaatatatcatca tgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactcca acatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccaga gttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatc aagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtg aaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctca ggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaag ctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttg gacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaata tggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa We get this part ,RBS-CmR, from BBa_P1004 by PCR. this doesn't have any promoters and terminators. false false _505_ 0 6371 9 It's complicated false We want to create promoterless CmR on pSB6A1. so We get this part ,RBS-CmR, from BBa_P1004 by PCR and then ligated with pSB6A1 false Kaneta Yusuke annotation2081191 1 RBS range2081191 1 6 9 annotation2081192 1 CmR range2081192 1 17 677 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K395303 1 PompC-CS1 PompC(CS1) Promoter regulated by phosphorylated OmpR 2010-10-01T11:00:00Z 2015-05-08T01:12:21Z ATTTACATTTTGAAACATCTGACCGTTGGATTATTCTGCATTTTTGGGGAGAATGGACT test false false _505_ 0 7027 9 Not in stock false test test false Taichi Nakamura annotation2102148 1 -10 range2102148 1 49 54 annotation2081250 1 Binding site1 range2081250 1 1 20 annotation2090359 1 -35 range2090359 1 26 31 BBa_C0040 1 tetR tetracycline repressor from transposon Tn10 (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999) Released HQ 2013 Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P> false true _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman. annotation23329 1 tetR range23329 1 4 620 annotation2213989 1 Help:Barcodes range2213989 1 661 685 annotation23330 1 SsrA range23330 1 621 654 BBa_K395700 1 BBa_K395700 crtW (beta-carotene ketolase) coding sequence 2010-10-04T11:00:00Z 2015-05-08T01:12:22Z Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Coding sequence for crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin. false false _505_ 0 6372 9 Not in stock false No special considerations. Please refer to Part BBa_K395706 for more information. false Yumiko Kinoshita BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2213986 1 Help:Barcodes range2213986 1 757 781 annotation1762 1 prefix range1762 1 1 2 annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation7039 1 BBa_C0062 range7039 1 1 756 annotation1765 1 A range1765 1 492 492 BBa_K118008_sequence 1 atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_C0078_sequence 1 atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc BBa_K395700_sequence 1 atgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga BBa_R1051_sequence 1 taacaccgtgcgtgttgacaattttacctctggcggtgatactggttgc BBa_K118002_sequence 1 atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa BBa_K118003_sequence 1 atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa BBa_K395603_sequence 1 aactttaagaaggagatatacatatgcaccatcatcatcatcattcttctggtctggtgccacgcggttctggtatgaaagaaaccgctgctgctaaattcgaacgccagcacatggacagcccagatctgggtaccggtggtggctccggtattgagggtcgcatgatgtccttttccgtactgcaagtgaaacgtctgcaaccggaactgattacaccggcaaaatcaacccctcaggaaaccaaatttctgagcgacatcgatgatcaagaatctctgcgcgttcagattccgattatcatgtgttataaagacaacccgagcctgaataaaaaccgtaacccggtcaaagccattcgtgaagcactgtcacgtgccctggtctattattatccactggccggtcgtctgcgtgaaggtccaaaccgtaaactggtggtcgattgtaacggcgaaggcatcctgtttgtggaagcttccgccgatgttacgctggaacagctgggggacaaaatcctgcctccttgtcctctgctggaggagtttctgtataacttcccgggttctgatggcattatcgactgcccactgctgctgattcaagtgacctgtctgacttgtggagggttcatcctggctctgcgcctgaaccatacaatgtgtgatgccgccggtctgctgctgtttctgaccgctattgccgaaatggcccgtggcgctcatgcccctagtattctgcctgtctgggagcgtgaactgctgtttgctcgtgatcctcctcgtatcacctgtgcccaccatgaatatgaggacgtgattggccactcagatgggtcttatgccagctcaaatcagagtaatatggtccaacgctcgttctattttggcgccaaagagatgcgcgtactgcgtaaacaaattccgccacacctgattagcacttgtagcaccttcgatctgatcaccgcttgtctgtggaaatgtcgtaccctggcactgaacattaacccgaaagaggccgttcgtgtttcgtgtattgtgaatgctcgtggtaaacacaacaacgttcgcctgcctctgggttattatggtaacgcctttgcctttccggcagcaatttcaaaagccgaacctctgtgtaaaaacccgctgggatatgctctggaactggtaaaaaaagcgaaagcgactatgaacgaagagtatctgcgttctgttgctgatctgctggtactgcgcggtcgccctcagtatagtagtaccggctcctatctgatcgttagcgacaacacccgtgtaggcttcggagatgtgaattttggctggggtcagcctgtttttgctggaccggttaaagcactggatctgatctcgttttatgtccaacacaaaaacaacaccgaggatggcattctggttccgatgtgtctgcctagtagcgctatggaacgctttcagcaggaactggagcgtattacccaggaaccaaaagaggacatctgtaacaatctgcgctcaacgtcccagtaaggctctaactctcctctggccatggcgatatcggatccaaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K395303_sequence 1 atttacattttgaaacatctgaccgttggattattctgcatttttggggagaatggact BBa_I742156_sequence 1 ctctaccggagaaatt BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I742157_sequence 1 atgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa BBa_K395601_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K395998_sequence 1 tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatttacattttgaaacatctgaccgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacga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ctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataatactagagctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataatactagagctcaaggaggtactagatgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttgatactagagaactttaagaaggagatatacatatgcaccatcatcatcatcattcttctggtctggtgccacgcggttctggtatgaaagaaaccgctgctgctaaattcgaacgccagcacatggacagcccagatctgggtaccggtggtggctccggtattgagggtcgcatgatgtccttttccgtactgcaagtgaaacgtctgcaaccggaactgattacaccggcaaaatcaacccctcaggaaaccaaatttctgagcgacatcgatgatcaagaatctctgcgcgttcagattccgattatcatgtgttataaagacaacccgagcctgaataaaaaccgtaacccggtcaaagccattcgtgaagcactgtcacgtgccctggtctattattatccactggccggtcgtctgcgtgaaggtccaaaccgtaaactggtggtcgattgtaacggcgaaggcatcctgtttgtggaagcttccgccgatgttacgctggaacagctgggggacaaaatcctgcctccttgtcctctgctggaggagtttctgtataacttcccgggttctgatggcattatcgactgcccactgctgctgattcaagtgacctgtctgacttgtggagggttcatcctggctctgcgcctgaaccatacaatgtgtgatgccgccggtctgctgctgtttctgaccgctattgccgaaatggcccgtggcgctcatgcccctagtattctgcctgtctgggagcgtgaactgctgtttgctcgtgatcctcctcgtatcacctgtgcccaccatgaatatgaggacgtgattggccactcagatgggtcttatgccagctcaaatcagagtaatatggtccaacgctcgttctattttggcgccaaagagatgcgcgtactgcgtaaacaaattccgccacacctgattagcacttgtagcaccttcgatctgatcaccgcttgtctgtggaaatgtcgtaccctggcactgaacattaacccgaaagaggccgttcgtgtttcgtgtattgtgaatgctcgtggtaaacacaacaacgttcgcctgcctctgggttattatggtaacgcctttgcctttccggcagcaatttcaaaagccgaacctctgtgtaaaaacccgctgggatatgctctggaactggtaaaaaaagcgaaagcgactatgaacgaagagtatctgcgttctgttgctgatctgctggtactgcgcggtcgccctcagtatagtagtaccggctcctatctgatcgttagcgacaacacccgtgtaggcttcggagatgtgaattttggctggggtcagcctgtttttgctggaccggttaaagcactggatctgatctcgttttatgtccaacacaaaaacaacaccgaggatggcattctggttccgatgtgtctgcctagtagcgctatggaacgctttcagcaggaactggagcgtattacccaggaaccaaaagaggacatctgtaacaatctgcgctcaacgtcccagtaaggctctaactctcctctggccatggcgatatcggatccaaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttgtactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac BBa_K395407_sequence 1 atttacattttgaaacatctgaccgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K395160_sequence 1 agctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa BBa_J23107_sequence 1 tttacggctagctcagccctaggtattatgctagc BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0034_sequence 1 aaagaggagaaa BBa_J15001_sequence 1 ctcaaggagg BBa_K082005_sequence 1 atgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaatga BBa_I742151_sequence 1 atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa BBa_I15010_sequence 1 atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctc BBa_K118012_sequence 1 gagg BBa_R0061_sequence 1 ttgacacctgtaggatcgtacaggtataat BBa_I15009_sequence 1 atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc BBa_K395604_sequence 1 aactttaagaaggagatatacatatgcaccatcatcatcatcattcttctggtctggtgccacgcggttctggtatgaaagaaaccgctgctgctaaattcgaacgccagcacatggacagcccagatctgggtaccggtggtggctccggtattgagggtcgcatgatgtccttttccgtactgcaagtgaaacgtctgcaaccggaactgattacaccggcaaaatcaacccctcaggaaaccaaatttctgagcgacatcgatgatcaagaatctctgcgcgttcagattccgattatcatgtgttataaagacaacccgagcctgaataaaaaccgtaacccggtcaaagccattcgtgaagcactgtcacgtgccctggtctattattatccactggccggtcgtctgcgtgaaggtccaaaccgtaaactggtggtcgattgtaacggcgaaggcatcctgtttgtggaagcttccgccgatgttacgctggaacagctgggggacaaaatcctgcctccttgtcctctgctggaggagtttctgtataacttcccgggttctgatggcattatcgactgcccactgctgctgattcaagtgacctgtctgacttgtggagggttcatcctggctctgcgcctgaaccatacaatgtgtgatgccgccggtctgctgctgtttctgaccgctattgccgaaatggcccgtggcgctcatgcccctagtattctgcctgtctgggagcgtgaactgctgtttgctcgtgatcctcctcgtatcacctgtgcccaccatgaatatgaggacgtgattggccactcagatgggtcttatgccagctcaaatcagagtaatatggtccaacgctcgttctattttggcgccaaagagatgcgcgtactgcgtaaacaaattccgccacacctgattagcacttgtagcaccttcgatctgatcaccgcttgtctgtggaaatgtcgtaccctggcactgaacattaacccgaaagaggccgttcgtgtttcgtgtattgtgaatgctcgtggtaaacacaacaacgttcgcctgcctctgggttattatggtaacgcctttgcctttccggcagcaatttcaaaagccgaacctctgtgtaaaaacccgctgggatatgctctggaactggtaaaaaaagcgaaagcgactatgaacgaagagtatctgcgttctgttgctgatctgctggtactgcgcggtcgccctcagtatagtagtaccggctcctatctgatcgttagcgacaacacccgtgtaggcttcggagatgtgaattttggctggggtcagcctgtttttgctggaccggttaaagcactggatctgatctcgttttatgtccaacacaaaaacaacaccgaggatggcattctggttccgatgtgtctgcctagtagcgctatggaacgctttcagcaggaactggagcgtattacccaggaaccaaaagaggacatctgtaacaatctgcgctcaacgtcccagtaaggctctaactctcctctggccatggcgatatcggatccaaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctga BBa_I742158_sequence 1 ctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa BBa_C0051_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I0462_sequence 1 aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_K395162_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K081016_sequence 1 attaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K274200_sequence 1 gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataatactagaggaggtactagatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa BBa_C0040_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_Q04400_sequence 1 aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I732100_sequence 1 atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa BBa_K395705_sequence 1 ctcaaggaggtactagatgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga BBa_I15008_sequence 1 atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z