BBa_K400000 1 BBa_K400000 composite heavy metal-detector(Zn, Cd, As) 2010-10-20T11:00:00Z 2015-05-08T01:12:23Z a soil bacterium It is a composite part of heavy metal-detecting promoters and reporter genes. ----PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)---- It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a capsule in order to be customized. false false _511_ 0 6409 9 It's complicated false Each of the parts were cleaved with Endonuclease and ligated with pSB1C3 vector. First, a promoter and a reporter gene were amplified by PCR and two of the pairs were ligated with pSB1C3 vector. Second, another promoter and reporter gene were put together by PCR and then ligated with pSB1C3 vector with the other two-pair-fragment. Three pairs were finally put in a vector and it underwent point mutation during the procedure because it had multiple cleavage sites. false Jonghwa Kim annotation2091111 1 BBa_B0034 range2091111 1 937 948 annotation2091120 1 polya range2091120 1 1784 1797 annotation2091113 1 BBa_E1010 range2091113 1 955 1635 annotation2091121 1 stop range2091121 1 1790 1790 annotation2091110 1 start range2091110 1 901 901 annotation2091114 1 mrfp1 range2091114 1 955 1629 annotation2091122 1 BBa_J04450 range2091122 1 729 1797 annotation2091109 1 LacI range2091109 1 894 928 annotation2091106 1 CAP range2091106 1 817 854 annotation2091116 1 BBa_B0010 range2091116 1 1669 1748 annotation2091104 1 BBa_E0040 range2091104 1 1 720 annotation2091118 1 BBa_B0012 range2091118 1 1757 1797 annotation2091112 1 conserved range2091112 1 941 944 annotation2091108 1 -10 range2091108 1 889 894 annotation2091115 1 barcode range2091115 1 1636 1660 annotation2091117 1 stem_loop range2091117 1 1680 1723 annotation2091123 1 mutated aryl acylamidase range2091123 1 1804 3297 annotation2091105 1 BBa_R0010 range2091105 1 729 928 annotation2091119 1 T7 TE range2091119 1 1764 1783 annotation2091103 1 GFP protein range2091103 1 1 720 annotation2091107 1 -35 range2091107 1 865 870 annotation2091124 1 BBa_K400628 range2091124 1 1804 3297 BBa_K400000_sequence 1 aaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattccttcatattgccgacaaagtacgcgttatccctgccggatgtggcgcgagcgccctatccggcatacacagatcgcatcaggttatgtttcgtttgtcatcaacctcaaatcaccaaaataatttattttatttcaataagatagataagtagaactgagaaagccatgctctcgtttcctaagagttgttgcattttgctatatgttacaatataacattacacatcatatacattaactctggaggaaactgttatgggtaagtcacattcgccagttcactggaagtcggcggccgagatcgtcgagttagtcaagagcaaacaaatttccccacgcgaggtcgtggaaagcacgatcgacctcatcgagcaacgcgatcccggcctgaatgcggtcgtgtacaaggcttacgatgaagcgcgcgaaaaagcggctgcgcttgagcggcgcatcatgcaaggcgagcctgttggcatgctggcgggggttccgactctcatgaaggacttgttcgcggccaagccgggctggccgtccacgctgggcggcattcgcgccctcaaggacgcccgcggcgctgccggcgtctggtccacttatccattgaagatgtccggcgaagacagcctgttgctgggacagacaaacagccccgtctatggctttcgcggaaccacggacaacacgtttttcggtccgacgcgcaatccattcaacctcgacttcaacgcaggggggtcttcgggaggagccgctgcgctcgtggccgatggcatcgttcctgtggcaggcggcacggatggcggcggctcgatccgcattccggcggcatggaccaatacctacggctttcagccttcgataggacgcgttccgttcaaaagcccgccccaatgctttccatccggggaccgtatctctatgaaggagcccatcacgcggacggtgccggatgcggcattggcaatgaatgtactgcacggcttcgaccgccgcgatcctgcttcgctgcgagtcaagctggacttcacctcagcgcttgcacaaggcgtgcgtggcaaaaaaatcggcctgacgctgaattacggggtatttcccgtccagcaagagattcaggatttgatcggcaaggctgcccgggtttttacagagcttggcgcacacgtcgagttcgtcgacctcggtatcccctacagccagaaacaaatgagcgatgcctggtgccgcatgatcgcgattcctactgtcgcctcgatgcaggcgctgcgcaaggaaggcattgacttatatggcgaacatcgggcagacatacccgatgcgctgatgaagtggatagatgcggtcgctgacataagcgtccagcagatcagcgctgatcaactattgagaacgaccgttttcgattgcatgaacggcgtattcgatcgctttgacctattattggcgccgaccctggcatgcatgcctgttcgcaatgcgactgatggctgcaccgagggaccaagccagatcaacggtgaagaaatcgaccctttgatcggctggtgcatgacttacctgaccaatttttcagggcatcccagtgccagtgtcccggctggcctgattgatggcctgccggcaggcatgctcattatcggcgatcgccaggcggacctggatgtcattgccgcaagcgccgctttcgagcgagccagcccgtggtcccaatactatgacattcccgccggacggcccctgtgataatctagtcgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaatgagaaaaaacttaaccggaggatgccatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaactagaccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggccttaactgcagtccggcaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z