LOCUS       BBa_K411113             1220 bp    DNA     linear       28-May-2024
DEFINITION  R0040+ B0034 + split_RFP_A+linker+split_eIF4A_A
ACCESSION   BBa_K411113
VERSION     BBa_K411113.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     CDS             543..592
                     /label=linker
     CDS             81..542
                     /label=RFP-split-A
     CDS             593..1217
                     /label=eIF4A-A
     misc_feature    1218..1220
                     /label=stop
     CDS             81..1220
                     /label=BBa_K411103
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggcagcag cggcagcagc ggcagcggca gcatggagcc ggaaggcgtc atcgagagta
      601 actggaacga gattgtggat agctttgatg acatgaatct ctcagagtcc ctcctccgtg
      661 gtatttatgc ctatggtttt gagaagccct ctgccatcca gcagcgagct attcttcctt
      721 gtatcaaggg ttatgatgtg attgctcaag cccagtctgg gactgggaaa acagctacat
      781 ttgccatatc aattctccag cagattgaat tagatctaaa ggccactcag gctttggttc
      841 tggcacccac acgtgaattg gctcagcaga tacaaaaggt ggttatggca ttaggagact
      901 acatgggtgc ctcttgtcat gcctgcattg ggggcaccaa tgtgcgtgct gaggtgcaga
      961 agctccagat ggaagctccc catatcatcg tgggtacccc tggccgggtg tttgacatgc
     1021 ttaaccggag atacctgtcc cccaaataca tcaagatgtt cgtactggat gaagcagatg
     1081 aaatgttaag ccgagggttc aaggatcaga tctatgacat attccagaag ctcaacagca
     1141 acacacaggt agttttgttg tctgctacaa tgccttctga tgtccttgag gtgaccaaga
     1201 aatttatgag agacccttga
//