BBa_K411115
1
BBa_K411115
pLac + eIF4A binding aptamer
2010-10-21T11:00:00Z
2015-05-08T01:12:25Z
Cloning
pLac (R0010) + eIF4A Binding Aptamer (K411105)
false
false
_526_
0
6636
9
It's complicated
false
none.
false
Yong-Yu, Jhan
component2101791
1
BBa_K411105
component2101783
1
BBa_R0010
annotation2101783
1
BBa_R0010
range2101783
1
1
200
annotation2101791
1
BBa_K411105
range2101791
1
209
268
BBa_K411105
1
BBa_K411105
eIF4A binding aptamer
2010-10-21T11:00:00Z
2015-05-08T01:12:25Z
no
no
false
false
_526_
0
6638
9
Not in stock
false
no
false
Geng Ming Su
annotation2094533
1
eIF4A binding aptamer
range2094533
1
1
60
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961227
1
start
range1961227
1
173
173
annotation1961223
1
CAP binding site
range1961223
1
89
126
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K411115_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagacactcggaggacagcttagatgcaaagccggagtgagtgtacaccccgcgccaggggaa
BBa_K411105_sequence
1
acactcggaggacagcttagatgcaaagccggagtgagtgtacaccccgcgccaggggaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z