BBa_K415039 1 BBa_K415039 pBR322 Derived Low Copy pMB1 Origin Operon 2010-10-25T11:00:00Z 2015-05-08T01:12:27Z PCR extract from pSB6A1 This origin is derived from the low (~20) copy pBR322 plasmid. The pMB1 replication origin for pBR322 is a regulated version of the high copy pUC19 origin (see I50022). The copy number is regulated by a small protein ROP. This part contains the origin with the appropriate copy-number regulatory domains, and can be used with the standard biobrick base vector I51020 to construct biobrick backbones. The part contains a RBS before the ROP protein but not a promotor. Note: This part contains an VR sequencing site between the ROP and origin, due to its homology with the pUC19 origin. This site should ideally be removed in a revised version. false false _525_ 0 6742 9 It's complicated false The extra reverse sequencing site in between the ROP and origin should be fixed in the future. Because of the sequence homologies with pUC19 origin backbones such as pSB1C3, it is impossible to specifically target the unwanted site with PCR without switching backbones. false Shawn Pan annotation2103568 1 pMB1 Ori range2103568 1 623 1237 annotation2103565 1 RBS range2103565 1 9 14 annotation2103567 1 Extra VR2 site range2103567 1 519 537 annotation2103566 1 ROP range2103566 1 19 212 BBa_K415039_sequence 1 ccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcccgctttatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtgaatcgcttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z