LOCUS       BBa_K415300             2494 bp    DNA     linear       01-Jun-2024
DEFINITION  Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt
ACCESSION   BBa_K415300
VERSION     BBa_K415300.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     promoter        38..43
                     /label=-10
     CDS             56..1183
                     /label=lacI-LVA
     misc_feature    56..1183
                     /label=BBa_C0012
     misc_feature    1145..1183
                     /label=LVA
     misc_feature    1184..1208
                     /label=barcode
     misc_feature    1217..1416
                     /label=BBa_R0010
     CDS             1217..1304
                     /label=end of LacI coding region (inactive)
     misc_feature    1305..1342
                     /label=CAP binding site
     promoter        1353..1358
                     /label=-35
     promoter        1377..1382
                     /label=-10
     misc_feature    1382..1416
                     /label=LacI binding site
     misc_feature    1389..1389
                     /label=start
     CDS             1426..2133
                     /label=cI lambda
     misc_feature    2134..2166
                     /label=LVA
     misc_feature    1423..2172
                     /label=BBa_C0051
     misc_feature    2173..2197
                     /label=barcode
ORIGIN
        1 gtcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
       61 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
      121 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
      181 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
      241 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
      301 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
      361 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
      421 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
      481 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
      541 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
      601 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
      661 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
      721 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
      781 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
      841 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
      901 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
      961 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     1021 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     1081 tcatgacgtc catccggccg tcctttgcat acaccatagg tgtggtttaa tttgatgccc
     1141 tttttcaggg ctggaatgtg taagagcggg gttatttatg ctgttgtttt tttgttactc
     1201 gggaagggct ttacctcttc cgcataaacg cttccatcag cgtttatagt taaaaaaatc
     1261 tttcggaact ggttttgcgc ttaccccaac caacagggga tttgctgctt tccattgagc
     1321 ctgtttctct gcgcgacgtt cgcggcggcg tgtttgtgca tccatctgga ttctcctgtc
     1381 agttagcttt ggtggtgtgt ggcagttgta gtcctgaacg aaaacccccc gcgattggca
     1441 cattggcagc taatccggaa tcgcacttac ggccaatgct tcgtttcgta tcacacaccc
     1501 caaagccttc tgctttgaat gctgcccttc ttcagggctt aatttttaag agcgtcacct
     1561 tcatggtggt cagtgcgtcc tgctgatgtg ctcattataa ccgccagtgg tatttatgtc
     1621 aacaccgcca gagataattt atcaccgcag atggttatct gtgcatgcat ttacgttgac
     1681 accatcgaat ggctgaaatg agctgttgac aattaatcat ccggctcgta taatgtgtgg
     1741 aattgtgagc ggataacaat ttcacacagg aaaccggtta tgagcacaaa aaagaaacca
     1801 ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa
     1861 aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc
     1921 gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca
     1981 aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag
     2041 atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt
     2101 tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg
     2161 gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa
     2221 ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt
     2281 ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt
     2341 gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta
     2401 aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc
     2461 gctagtcagt ggcctgaaga gacgtttggc tgat
//