BBa_K415300 1 Toggle Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt 2010-10-22T11:00:00Z 2015-05-08T01:12:27Z Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU. Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.The Collins UV Toggle (2000) biobricked. It is a bistable toggle (on or off state) and switching is induced by UV exposure or IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc. false false _525_ 0 6736 9 It's complicated false We used PCR to biobrick this sequence from its original form in an Amp plasmid with SC101 origin. We had to transfer into pSB1C3 to submit it to the registry. false Leanna Morinishi, Grant Robinson, Andrew Yang, Paul Muir, Shirley Galbiati, Shawn Pan, Jason Stevens, Lauren McGough, Deepak Mishra, Ron Weiss annotation2093364 1 LVA range2093364 1 2134 2166 annotation2093349 1 -35 range2093349 1 15 20 annotation2093361 1 LacI binding site range2093361 1 1382 1416 annotation2093350 1 OR1 range2093350 1 25 41 annotation2093352 1 lacI-LVA range2093352 1 56 1183 annotation2093351 1 -10 range2093351 1 38 43 annotation2093358 1 CAP binding site range2093358 1 1305 1342 annotation2093365 1 BBa_C0051 range2093365 1 1423 2172 annotation2093354 1 LVA range2093354 1 1145 1183 annotation2093356 1 BBa_R0010 range2093356 1 1217 1416 annotation2093363 1 cI lambda range2093363 1 1426 2133 annotation2093347 1 OR2 range2093347 1 1 17 annotation2093360 1 -10 range2093360 1 1377 1382 annotation2093362 1 start range2093362 1 1389 1389 annotation2093359 1 -35 range2093359 1 1353 1358 annotation2093357 1 end of LacI coding region (inactive) range2093357 1 1217 1304 annotation2093355 1 barcode range2093355 1 1184 1208 annotation2093366 1 barcode range2093366 1 2173 2197 annotation2093348 1 BBa_R0051 range2093348 1 1 49 annotation2093353 1 BBa_C0012 range2093353 1 56 1183 BBa_K415300_sequence 1 gtcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcatgacgtccatccggccgtcctttgcatacaccataggtgtggtttaatttgatgccctttttcagggctggaatgtgtaagagcggggttatttatgctgttgtttttttgttactcgggaagggctttacctcttccgcataaacgcttccatcagcgtttatagttaaaaaaatctttcggaactggttttgcgcttaccccaaccaacaggggatttgctgctttccattgagcctgtttctctgcgcgacgttcgcggcggcgtgtttgtgcatccatctggattctcctgtcagttagctttggtggtgtgtggcagttgtagtcctgaacgaaaaccccccgcgattggcacattggcagctaatccggaatcgcacttacggccaatgcttcgtttcgtatcacacaccccaaagccttctgctttgaatgctgcccttcttcagggcttaatttttaagagcgtcaccttcatggtggtcagtgcgtcctgctgatgtgctcattataaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtgcatgcatttacgttgacaccatcgaatggctgaaatgagctgttgacaattaatcatccggctcgtataatgtgtggaattgtgagcggataacaatttcacacaggaaaccggttatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z