BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K424005
1
BBa_K424005
RBS + LucC + Terminator
2010-06-06T11:00:00Z
2015-05-08T01:12:28Z
This part was based on the basic part <partinfo>BBa_K424004</partinfo> created by the Panama 2010 iGEM team.
What does the device do? Helps to produce light
What are the inputs and outputs to the device? Takes in a long-chain aldehyde + CoA + NADP(+) and produces a long-chain acyl-CoA + NADPH.
Are any other devices needed? Also requires the other genes of the bacterial luciferase pathway.
Does the device have any chassis dependencies?
true
false
_545_
0
6272
9
Discontinued
false
This only one of five required steps... look up Lux AB DE.
false
Patrick Nee
component2070331
1
BBa_B0010
component2070333
1
BBa_B0012
component2070325
1
BBa_B0030
component2070330
1
BBa_K424004
annotation2070325
1
BBa_B0030
range2070325
1
1
15
annotation2070330
1
BBa_K424004
range2070330
1
24
275
annotation2070333
1
BBa_B0012
range2070333
1
372
412
annotation2070331
1
BBa_B0010
range2070331
1
284
363
BBa_K424004
1
BBa_K424004
LuxC gene from V. fischeri for generating light.
2010-06-06T11:00:00Z
2015-05-08T01:12:28Z
ACCESSION Y00509 REGION: 1624..1866
AUTHORS Engebrecht,J. and Silverman,M.
TITLE Nucleotide sequence of the regulatory locus controlling expression
of bacterial genes for bioluminescence
JOURNAL Nucleic Acids Res. 15 (24), 10455-10467 (1987)
PUBMED 3697093
Which species is the enzyme from? Vibrio fischeri
What reaction does it catalyze? One of five steps in the production of light.
Does it work in E. coli? Yes, several groups have used this operon as a reporter in E. coli.
Does it require any other parts? Yes. The full luxCDABE operon is needed for light production.
true
false
_545_
0
6272
9
Discontinued
false
Requires the other four steps in the LuxABCDE process
false
Patrick Nee
annotation2070322
1
Start
range2070322
1
23
29
annotation2070321
1
TaaTaa
range2070321
1
246
252
annotation2070323
1
LuxC protien
range2070323
1
35
224
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K424005_sequence
1
attaaagaggagaaatactagagataggttcgtcgccgattatattcttcacttttccatcgttgaccaacggtatataaaaaattcacaatctgatttaaatttagattaattctatctttgatatttaatgtttttgaaactgaactttcagtaatgacagataattttactctattatcattatttagtttaacttctttatatgcataattatcaaaatcttgaatcattccattaattatcattggaatacatttattcattaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K424004_sequence
1
ataggttcgtcgccgattatattcttcacttttccatcgttgaccaacggtatataaaaaattcacaatctgatttaaatttagattaattctatctttgatatttaatgtttttgaaactgaactttcagtaatgacagataattttactctattatcattatttagtttaacttctttatatgcataattatcaaaatcttgaatcattccattaattatcattggaatacatttattcattaataataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z