LOCUS BBa_K424017 3348 bp DNA linear 14-Jun-2024 DEFINITION Test plataform for rhamnosyltransferase BioBrick (Rh1AB_BB) expression in E. coli ACCESSION BBa_K424017 VERSION BBa_K424017.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 CDS 152..939 /label=RhlA CDS 1105..2385 /label=RhlB misc_feature 871..876 /label=silent mutation misc_feature 1894..1899 /label=silent mutation misc_feature 2008..2013 /label=silent mutation misc_feature 52..54 /label=start codon cysteine misc_feature 2483..2485 /label=end codon alanine misc_feature 152..2385 /label=RhlAB Panama misc_feature 52..2485 /label=Rhamnosyltransferase complex misc_feature 52..2485 /label=BBa_K424018 CDS 2492..3211 /label=GFP protein CDS 2492..3211 /label=BBa_E0040 stem_loop 3231..3274 /label=stem_loop misc_feature 3220..3299 /label=BBa_B0010 terminator 3220..3299 /label=BBa_B0010 stem_loop 3315..3334 /label=T7 TE misc_feature 3341..3341 /label=stop polyA_site 3335..3348 /label=polya misc_feature 3308..3348 /label=BBa_B0012 terminator 3308..3348 /label=BBa_B0012 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga gtgtctgccg 61 ttcgaacaac aagaacgaac tctacgtaat gccgggatac ccgtggcagc gatagctgtt 121 tgcctgttcg aaaatttttg ggaggtgtga aatgcggcgc gaaagtctgt tggtatcggt 181 ttgcaagggc ctgcgggtac atgtcgagcg cgttgggcag gatcccgggc gcagcacggt 241 gatgctggtc aacggcgcga tggcgaccac cgcctcgttc gcccggacct gcaagtgcct 301 ggccgaacat ttcaacgtgg tgctgttcga cctgcccttc gccgggcagt cgcgtcagca 361 caacccgcag cgcgggttga tcaccaagga cgacgaggtg gaaatcctcc tggcgctgat 421 cgagcgcttc gaggtcaatc acctggtctc cgcgtcctgg ggcggtatct ccacgctgct 481 ggcgctgtcg cgcaatccgc gcggcatccg cagctcggtg gtgatggcat tcgcccctgg 541 actgaaccag gcgatgctcg actacgtcgg gcgggcgcag gcgctgatcg agctggacga 601 caagtcggcg atcggccatc tgctcaacga gaccgtcggc aaatacctgc cgccgcgcct 661 gaaagccagc aaccatcagc acatggcttc gctggccacc ggcgaatacg agcaggcgcg 721 ctttcacatc gaccaggtgc tggcgctcaa cgatcggggc tacctggctt gcctggagcg 781 gatccagagc cacgtgcatt tcatcaacgg cagctgggac gaatacacca ccgccgagga 841 cgcccgccag ttccgcgact acctgccgca ctgtagtttc tcgcgggtgg agggcaccgg 901 gcatttcctc gacctggagt ccaagctggc cgcggtacgc gtgcaccgcg ccctgctcga 961 gcacctgctg aagcaaccgg agccgcagcg ggcggaacgc gcggcgggat tccacgagat 1021 ggccatcggc tacgcctgaa cccttgacct gcgaagaccc ggcctggccg ggctttgcgg 1081 ttgcataacg cacggagtag ccccatgcac gccatcctca tcgccatcgg ctcggccggc 1141 gacgtatttc ccttcatcgg cttggcccgg accctgaaat tgcgcgggca ccgcgtgagc 1201 ctctgcacca tcccggtgtt tcgcgacgcg gtggagcagc acggcatcgc gttcgtcccg 1261 ctgagcgacg aactgaccta ccgccgggcc atgggcgatc cgcgcctgtg ggaccccaag 1321 acgtccttcg gcgtgctctg gcaagccatc gccgggatga tcgagccggt ctacgagtac 1381 gtctcggcgc agcgccatga cgacatcgtg gtggtcggct cgctctgggc gctgggcgca 1441 cgcatcgctc acgagaagta cgggattccc tacctgtccg cgcaggtctc gccatcgacc 1501 ttgctgtcgg cgcacctgcc gccggtacac cccaagttca acgtgcccga gcagatgccg 1561 ctggcgatgc gcaagctgct ctggcgctgc atcgagcgct tcaagctgga tcgcacctgc 1621 gcgccggaga tcaacgcggt gcgccgcaag gtcggcctgg agacgccggt gaagcgcatc 1681 ttcacccaat ggatgcattc gccgcagggc gtggtctgcc tgttcccggc ctggttcgcg 1741 ccaccccagc aggattggcc gcaacccctg cacatgaccg gcttcccgct gttcgacggc 1801 agtatcccgg ggaccccgct cgacgacgaa ctgcaacgct ttctcgatca gggcagccgg 1861 ccgctggtgt tcacccaggg ctcgaccgaa cacctacagg gcgacttcta cgccatggcc 1921 ctgcgcgcgc tggaacgcct cggcgcgcgt gggatcttcc tcaccggcgc cggccaggaa 1981 ccgctgcgcg gcttgccgaa tcacgtgcta cagcgcgcct acgcgccact gggagccttg 2041 ctgccatcgt gcgccgggct ggtccatccg ggcggtatcg gcgccatgag cctggccttg 2101 gcggcggggg tgccgcaggt gctgctgccc tgcgcccacg accagttcga caatgccgaa 2161 cggctggtcc ggctcggctg cgggatgcgc ctgggcgtgc cgttgcgcga gcaggagttg 2221 cgcggggcgc tgtggcgctt gctcgaggac ccggccatgg cggcggcctg tcggcgtttc 2281 atggaattgt cacaaccgca cagtatcgct tgcggtaaag cggcccaggt ggtcgaacgt 2341 tgtcataggg agggggatgc gcgatggctg aaggctgcgt cctgaacggt gctggcataa 2401 cagatagggt tgccatgatt ttgccgtatc ggcaaggctg cgcgcttgac agcgtcatac 2461 cccgggccaa ttctgctgtg atgcatacta gatgcgtaaa ggagaagaac ttttcactgg 2521 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 2581 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 2641 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 2701 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 2761 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 2821 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 2881 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 2941 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 3001 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 3061 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 3121 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 3181 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa 3241 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3301 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //