LOCUS BBa_K427009 5138 bp DNA linear 11-May-2024 DEFINITION Intelligent arabinose biosensor with differential response ACCESSION BBa_K427009 VERSION BBa_K427009.1 FEATURES Location/Qualifiers stem_loop 8..27 /label=T7 TE misc_feature 34..34 /label=stop polyA_site 28..41 /label=polya misc_feature 1..41 /label=BBa_B0012 terminator 1..41 /label=BBa_B0012 stem_loop 62..84 /label=stem_loop misc_feature 50..95 /label=BBa_B0011 terminator 50..95 /label=BBa_B0011 terminator 1..95 /label=BBa_B0014 promoter 104..234 /label=promoter misc_feature 143..160 /label=AraI1 misc_feature 164..181 /label=AraI2 promoter 104..233 /label=BBa_K206000 RBS 242..253 /label=RBS CDS 260..646 /label=MuMor misc_feature 242..646 /label=BBa_K427002 promoter 655..784 /label=promoter promoter 655..784 /label=BBa_J44002 stem_loop 800..819 /label=T7 TE misc_feature 826..826 /label=stop polyA_site 820..833 /label=polya misc_feature 793..833 /label=BBa_B0012 terminator 793..833 /label=BBa_B0012 stem_loop 854..876 /label=stem_loop misc_feature 842..887 /label=BBa_B0011 terminator 842..887 /label=BBa_B0011 terminator 793..887 /label=BBa_B0014 promoter 896..1025 /label=BBa_I13453 misc_feature 1034..1045 /label=B0034 CDS 1055..1297 /label=phiR73 delta misc_feature 1055..1055 /label=phiR73 delta misc_feature 1135..1135 /label=silent mutation to remove PstI site misc_feature 1034..1297 /label=BBa_I746352 promoter 1306..1435 /label=PBad Weak Reverse promoter 1306..1435 /label=BBa_K427000 stem_loop 1451..1470 /label=T7 TE misc_feature 1477..1477 /label=stop polyA_site 1471..1484 /label=polya misc_feature 1444..1484 /label=BBa_B0012 terminator 1444..1484 /label=BBa_B0012 stem_loop 1505..1527 /label=stem_loop misc_feature 1493..1538 /label=BBa_B0011 terminator 1493..1538 /label=BBa_B0011 terminator 1444..1538 /label=BBa_B0014 promoter 1547..1677 /label=promoter misc_feature 1586..1603 /label=AraI1 misc_feature 1607..1624 /label=AraI2 promoter 1547..1676 /label=BBa_K206001 RBS 1685..1696 /label=RBS CDS 1702..2122 /label=MuC misc_feature 1685..2122 /label=BBa_K427001 stem_loop 2138..2157 /label=T7 TE misc_feature 2164..2164 /label=stop polyA_site 2158..2171 /label=polya misc_feature 2131..2171 /label=BBa_B0012 terminator 2131..2171 /label=BBa_B0012 stem_loop 2192..2214 /label=stem_loop misc_feature 2180..2225 /label=BBa_B0011 terminator 2180..2225 /label=BBa_B0011 terminator 2131..2225 /label=BBa_B0014 promoter 2234..2304 /label=Pm promoter 2234..2304 /label=BBa_K427003 RBS 2313..2325 /label=RBS-3\Weak RBS 2319..2322 /label=RBS misc_feature 2313..2325 /label=BBa_B0032 RBS 2313..2325 /label=BBa_B0032 CDS 2332..3051 /label=GFP protein CDS 2332..3051 /label=BBa_E0040 stem_loop 3071..3114 /label=stem_loop misc_feature 3060..3139 /label=BBa_B0010 terminator 3060..3139 /label=BBa_B0010 stem_loop 3155..3174 /label=T7 TE misc_feature 3181..3181 /label=stop polyA_site 3175..3188 /label=polya misc_feature 3148..3188 /label=BBa_B0012 terminator 3148..3188 /label=BBa_B0012 misc_feature 2313..3188 /label=BBa_E0240 promoter 3197..3288 /label=PO promoter 3197..3288 /label=BBa_I746361 misc_feature 3301..3304 /label=conserved RBS 3297..3308 /label=BBa_B0034 CDS 3315..4037 /label=BBa_E0030 stem_loop 4057..4100 /label=stem_loop misc_feature 4046..4125 /label=BBa_B0010 terminator 4046..4125 /label=BBa_B0010 stem_loop 4141..4160 /label=T7 TE misc_feature 4167..4167 /label=stop polyA_site 4161..4174 /label=polya misc_feature 4134..4174 /label=BBa_B0012 terminator 4134..4174 /label=BBa_B0012 misc_feature 3297..4174 /label=BBa_E0430 promoter 4183..4261 /label=Pmom promoter 4183..4261 /label=BBa_K427004 misc_feature 4274..4277 /label=conserved RBS 4270..4281 /label=BBa_B0034 CDS 4288..4998 /label=mCherry misc_feature 4639..4639 /label=C->T (removing PstI site) misc_feature 4288..5001 /label=BBa_J06504 stem_loop 5021..5064 /label=stem_loop misc_feature 5010..5089 /label=BBa_B0010 terminator 5010..5089 /label=BBa_B0010 stem_loop 5105..5124 /label=T7 TE misc_feature 5131..5131 /label=stop polyA_site 5125..5138 /label=polya misc_feature 5098..5138 /label=BBa_B0012 terminator 5098..5138 /label=BBa_B0012 misc_feature 4270..5138 /label=BBa_J06702 ORIGIN 1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 61 aaagccagat tattaatccg gcttttttat tattttacta gagacattga ttatttgcac 121 ggcgtcacac tttgctatgc catagcaaga tagtccataa gattagcgga tcctacctga 181 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga 241 gaaagaggag aaatactaga tgactgaaga cctgtttggt gatctgcaag acgataccat 301 tctggcacac ctggataatc ctgccgagga cacaagccgt tttccggcgc tgctggctga 361 actgaacgat ctgctgcgtg gtgagctgtc tcgcctggga gttgaccctg cccattctct 421 ggagattgtc gtggccattt gtaaacatct gggcggaggc caagtttata ttccacgtgg 481 acaggcactg gacagtctga ttcgtgacct gcgcatttgg aacgatttca acgggcgtaa 541 tgtgagtgaa ctgactaccc gctatggagt gacttttaac accgtgtata aagccattcg 601 ccgtatgcgt cgtctgaaat atcgccagta tcaaccgagc ctgctgtact agaggctagc 661 ccaaaaaaac ggtatggaga aacagtagag agttgcgata aaaagcgtca ggtaggatcc 721 gctaatctta tggataaaaa tgctatggca tagcaaagtg tgacgccgtg caaataatca 781 atgttactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 841 gagagaatat aaaaagccag attattaatc cggctttttt attattttac tagagacatt 901 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg 961 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccg tttttttggg 1021 ctagctacta gagaaagagg agaaatacta gatgatgcgc tgccctttct gtcgtcattc 1081 agcgcatacc cgcaccagcc ggtatgtgag tgacaatgtc aaagaaagtt atctccagtg 1141 ccagaatatt tactgttcgg cgacatttaa aacgcatgag tcaatttgtg ccgtgattcg 1201 ttctccggtc acggaggaaa aaccagcacc ggcaagcaca gcaccggctg ttgtccgaaa 1261 agttaaaggc tgttacagct caccattcaa ccattaatac tagaggctag cccaaaaaaa 1321 cggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt 1381 atggataaaa aagctatggc atagcaaagt gtgacgccgt gcaaataatc aatgttacta 1441 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata 1501 taaaaagcca gattattaat ccggcttttt tattatttta ctagagacat tgattatttg 1561 cacggcgtca cactttgcta tgccatagct tttttatcca taagattagc ggatcctacc 1621 tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg gctagctact 1681 agagaaagag gagaaatact agatgcaaca tgacctgttt gagcatgatc cggcgattcg 1741 tcagctgatt ggccatatcg acaacattcc ggcacctgaa ctggaaagtc gctggcctcg 1801 tagcgtggtt gatctgatcg atgttctgga gaacgaactg aaacgccaaa atgtgtctaa 1861 cccacgtgag ctggctcgta aacaagcagt tgccctgtct tgcttcctgg gtggacgtca 1921 attctatatc ccgtgtggcg acacgatcct gacagcactg cgtgatgatc tgctgtattg 1981 ccagtttaat ggccgtaaca tggaagaact gcgccgtcaa tatcgtctgt ctcagccaca 2041 gatttatcaa atcattgctc gccagcgtaa actgcataca cgtcgccatc aacctgacct 2101 gttctctccg gaaacaccga aatactagag tcacactggc tcaccttcgg gtgggccttt 2161 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat 2221 tattttacta gagttctgta aacagtaaag ccggttaatc cggctttttt tacgtcctca 2281 atatcctgtg atgaataacc gtactactag agtcacacag gaaagtacta gatgcgtaaa 2341 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 2401 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 2461 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 2521 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 2581 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 2641 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 2701 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 2761 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt 2821 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa 2881 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca 2941 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt 3001 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc 3061 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3121 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3181 cgtttatata ctagagcgcg ccccgcgatt cgctaaggtg ctgttgtgtc agtgataagc 3241 catccgggac tgatggcgga ggatgcgcat cgtcgggaaa ctgatgccta ctagagaaag 3301 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 3361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 3421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 3481 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc 3541 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 3601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 3661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 3721 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 3781 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 3841 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 3901 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc 3961 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 4021 agctgtacaa gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 4081 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 4141 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agggtaatac agatcgatta 4201 tgccccaata accacactca acccatgatg ttttttaaga tagtggcgaa ttgatgcaaa 4261 gtactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga taacatggcc 4321 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 4381 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 4441 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 4501 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 4561 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 4621 gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 4681 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 4741 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 4801 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 4861 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 4921 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 4981 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 5041 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 5101 cactggctca ccttcgggtg ggcctttctg cgtttata //