BBa_K511203 1 BBa_K511203 rtTA3 Tetracycline-Inducible Transactivator MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This protein is a heavily engineered fusion between the tetracycline operator binding domain from TetR and the viral VP16 transactivation domain from the herpes simplex virus. This part encodes a transactivator composed of the DNA-binding domain for the tetracycline operator sequence and the viral VP16 transactivation domain; this protein has been engineered over previous versions to be more sensitive to tetracycline and its analogues. Figures 1 and 2 below show expression from a promoter with six tetracycline response elements (TRE-Tight) in the presence of rtTA3 and various levels of doxycycline, a tetracycline analogue. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2143727 1 rtTA3 range2143727 1 1 708 BBa_K511203_sequence 1 atgtctaggctggacaagagcaaagtcataaacggagctctggaattactcaatggtgtcggtatcgaaggcctgacgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacgtgaagaacaagcgggccctgctcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactttctgcggaacaacgccaagtcataccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcacccgcccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagaggaaagagagacacctaccaccgattctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatatgtggcctggagaaacagctaaagtgcgaaagcggcgggccgaccgacgcccttgacgattttgacttagacatgctcccagccgatgcccttgacgattttgaccttgacatgctccccgggtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z