BBa_K511205 1 BBa_K511205 LacI-KRAB Repressor MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This protein is a fusion between a mammalian codon-optimized version of the LacI repressor originally derived from Escherichia coli and the Kruppel-associated box derived from a human zinc finger repressor. This part encodes a repressor protein that binds tightly to the lactose operator originating from Escherichia coli. This protein is a fusion between the LacI repressor and the Kruppel-associated box (KRAB), a domain previously shown to strongly repress transcription from affected promoters via chromatin silencing. This repressor has been codon-optimized for mammalian expression and is capable of exerting very strong repression on promoters with internal lactose operator sites. Because LacI-KRAB-mediated repression can last for days due to its chromatin remodeling activity, LacI-KRAB is recommended for situations in which a change in transcriptional activity must be stable for extended periods of time, such as a permanent switch. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2143829 1 LacI range2143829 1 1 1080 annotation2143831 1 KRAB Domain range2143831 1 1081 1464 BBa_K511205_sequence 1 atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagccaaaaaagaagagaaaggtcgacggcggtggtgctttgtctcctcagcactctgctgtcactcaaggaagtatcatcaagaacaaggagggcatggatgctaagtcactaactgcctggtcccggacactggtgaccttcaaggatgtatttgtggacttcaccagggaggagtggaagctgctggacactgctcagcagatcgtgtacagaaatgtgatgctggagaactataagaacctggtttccttgggttatcagcttactaagccagatgtgatcctccggttggagaagggagaagagccctggctggtggagagagaaattcaccaagagacccatcctgattcagagactgcatttgaaatcaaatcatcagtttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z