LOCUS       BBa_K511810             3130 bp    DNA     linear       20-May-2024
DEFINITION  Repressible Tango Receptor Generator (Hef1a-LacO-AVPR2-TEVs-Gal4-VP
            16) MammoBlock Device
ACCESSION   BBa_K511810
VERSION     BBa_K511810.1  
FEATURES             Location/Qualifiers
     promoter        1..1275
                     /label=Hef1-alpha Promoter
     misc_feature    201..221
                     /label=Lac O1
     misc_feature    272..291
                     /label=Lac Oid
     promoter        1..1275
                     /label=BBa_K511000
     misc_feature    1277..1297
                     /label=attB1
     misc_feature    1276..1297
                     /label=BBa_J85600
     CDS             1298..2410
                     /label=AVPR2 Receptor 
     misc_feature    2411..2449
                     /label=Flexible TEV Recognition Sequence
     CDS             2450..3127
                     /label=Gal4-VP16 Transactivator
     misc_feature    1298..3130
                     /label=BBa_K511400
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaagctag aattgtgagc ggataacaat tccacagtcg accctaggtt
      241 gtgtcgcgag tgttggatcc cagctgacac caattgtgag cgctcacaat tgctagccgt
      301 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
      361 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
      421 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
      481 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
      541 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
      601 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
      661 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
      721 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
      781 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
      841 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
      901 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
      961 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     1021 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     1081 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     1141 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     1201 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     1261 tcttccattt caggtccaag tttgtacaaa aaagcagatg ctcatggcgt ccaccacttc
     1321 cgctgtgcct gggcatccct ctctgcccag cctgcccagc aacagcagcc aggagaggcc
     1381 actggacacc cgggacccgc tgctagcccg ggcggagctg gcgctgctct ccatagtctt
     1441 tgtggctgtg gccctgagca atggcctggt gctggcggcc ctagctcggc ggggccggcg
     1501 gggccactgg gcacccatac acgtcttcat tggccacttg tgcctggccg acctggccgt
     1561 ggctctgttc caagtgctgc cccagctggc ctggaaggcc accgaccgct tccgtgggcc
     1621 agatgccctg tgtcgggccg tgaagtatct gcagatggtg ggcatgtatg cctcctccta
     1681 catgatcctg gccatgacgc tggaccgcca ccgtgccatc tgccgtccca tgctggcgta
     1741 ccgccatgga agtggggctc actggaaccg gccggtgcta gtggcttggg ccttctcgct
     1801 ccttctcagc ctgccccagc tcttcatctt cgcccagcgc aacgtggaag gtggcagcgg
     1861 ggtcactgac tgctgggcct gctttgcgga gccctggggc cgtcgcacct atgtcacctg
     1921 gattgccctg atggtgttcg tggcacctac cctgggtatc gccgcctgcc aggtgctcat
     1981 cttccgggag attcatgcca gtctggtgcc agggccatca gagaggcctg gggggcgccg
     2041 caggggacgc cggacaggca gccccggtga gggagcccac gtgtcagcag ctgtggccaa
     2101 gactgtgagg atgacgctag tgattgtggt cgtctatgtg ctgtgctggg cacccttctt
     2161 cctggtgcag ctgtgggccg cgtgggaccc ggaggcacct ctggaagggg cgccctttgt
     2221 gctactcatg ttgctggcca gcctcaacag ctgcaccaac ccctggatct atgcatcttt
     2281 cagcagcagc gtgtcctcag agctgcgaag cttgctctgc tgtgcccggg gacgcacccc
     2341 acccagcctg ggtccccaag atgagtcctg caccaccgcc agctcctccc tggccaagga
     2401 cacttcatcg ggatccggag agaacctgta cttccagctc ggatccggag cccccccgac
     2461 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
     2521 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     2581 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     2641 gcagatgttt accgatgccc ttggaattga cgagtacggt gggacgcgta tgaagctact
     2701 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga
     2761 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac
     2821 caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact
     2881 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga
     2941 ttctttacag gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa
     3001 agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca
     3061 gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt
     3121 gactgtataa
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