BBa_K511826 1 BBa_K511826 Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent components. This MammoBlock composite device produces both the N-Cadherin cell-cell adhesion compound and the yellow fluorescent protein EYFP when induced with Gal4 transactivator variants and otherwise produces both proteins at a low, basal (OFF) level. This generator is particularly well suited to the engineering of cellular patterns given the combination of adhesion and fluorescent readout. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146322 1 BBa_K511600 component2146316 1 BBa_K511003 component2146318 1 BBa_J85600 annotation2146318 1 BBa_J85600 range2146318 1 221 242 annotation2146322 1 BBa_K511600 range2146322 1 243 3756 annotation2146316 1 BBa_K511003 range2146316 1 1 220 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511003 1 BBa_K511003 UAS-Gal4 Promoter MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter. This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise. As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator. false false _674_ 0 6719 9 It's complicated true Not applicable. false Grant Robinson annotation2139553 1 Major-Late Adenovirus Promoter range2139553 1 160 200 annotation2139552 1 Gal4-Binding Region range2139552 1 46 148 BBa_K511600 1 BBa_K511600 N-Cadherin-2A-EYFP Cell Adhesion MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z The N-Cadherin sequence of this part was isolated from a murine source; the viral 2A self-cleaving peptide was isolated from a virus with homology to the foot-and-mouth disease virus; the EYFP fluorescent reporter is an engineered version of the original green fluorescent protein from Aequorea victoria. This part encodes a protein kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. This part additionally encodes an enhanced yellow fluorescent protein (EYFP) linked to the N-Cadherin by a self-cleaving viral 2A peptide, allowing for bicistronic expression of both a cell adhesion protein and an identifying marker with a single part. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2144668 1 2A Self-Cleaving Peptide range2144668 1 2719 2794 annotation2144667 1 Murine N-Cadherin range2144667 1 1 2718 annotation2144670 1 EYFP range2144670 1 2719 3511 BBa_K511826_sequence 1 gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgaccccaagtttgtacaaaaaagcagatgtgccggatagcgggagcgccgcggaccctgctgccgcttctggcggccttgcttcaggcgtctgtggaggcttctggtgaaattgcattatgcaagactggatttcctgaagatgtttacagcgcagtcttaccgaaggatgtgcacgaaggacagccccttctcaatgtgaaattcagcaactgcaatagaaaaaggaaagttcagtatgaaagcagcgagccagcagatttcaaggtggacgaggacggcacggtgtatgctgtgagaagcttccctctcactgcagagcaggcaaagttcctgatatatgcccaagacaaagaaacccaggaaaagtggcaggtagctgtaaacctgagccgggagccaaccctgactgaggagcctatgaaggaaccacatgaaattgaagaaatagtattccctagacaacttgccaagcacagtggagctctacaaaggcagaagagagactgggtcatcccgccaatcaacttgccagaaaactccagaggaccctttcctcaagagcttgtcagaatcaggtctgatagagataaaaacctttccctgagatacagcgtcactgggccaggagctgaccagcctccaacgggcatcttcattatcaaccccatctcaggacagctgtcagtcacaaagcctctggatcgagagctgatagcccggtttcacttgagagcacatgcagtggacatcaatggcaatcaagtggagaaccccattgacattgtcatcaatgttattgacatgaatgataacagacctgagtttctgcaccaggtttggaatgggtctgttccagagggatcaaagcctgggacgtatgtgatgacggtcactgccattgatgcggatgatccaaatgccctgaatggaatgctgcggtacaggatcctgtcccaggcgcccagcacaccttcacccaacatgtttacaatcaacaatgagactggggacatcatcactgtggcagctggtctggatcgagagaaagtgcaacagtatacgttaataattcaagccacagacatggaaggcaatcccacttatggcctttcaaacacagccacagccgtcatcacggtgacagatgtcaatgacaatcctccagagtttactgccatgactttctacggagaagtccctgagaacagggtggacgtcattgtagccaacctaactgtcacggacaaagatcagccccacacgccggcctggaatgcggcatacagaatcagtggtggagaccctacaggaaggtttgccatcctgacagaccccaacagcaatgatgggctagtcacagtggtaaaaccaattgactttgaaacgaataggatgtttgtccttactgttgctgcagaaaaccaagtgccattagctaaaggcattcagcacccacctcagtcgacagccactgtgtctgtgacagttattgatgtcaatgaaaatccttattttgccccaaatcctaaaatcattcgccaagaggaaggcctccacgcaggtaccatgctgaccacgctcactgctcaggaccccgatcgatatatgcaacagaatatcagatacacaaaattgtctgatcctgccaactggctgaaaatagaccccgtgaatgggcagatcactactattgccgttttggacagagaatcgccaaatgtaaaaaacaacatctataatgctaccttccttgcttctgacaatggaatcccgcctatgagtgggacaggaacactgcaaatctatttacttgatatcaatgacaacgcccctcaggtgttacctcaagaggcagagacctgtgaaactccagaacccaactcaattaacatcacagcacttgattatgacatagacccaaacgccgggccattcgcgtttgatcttcccttatctccagtgactattaaaagaaactggaccatcaaccggcttaatggtgattttgctcagctcaatttaaagataaaatttttggaagctggtatctatgaagttcccatcattatcacagattcagggaatccccccaagtccaacatttccatcctgcgtgtgaaagtttgccagtgtgactccaatggagactgcacggacgtggacaggatcgtgggtgcagggcttggcacgggtgccatcatcgctatccttctgtgtatcatcatcctgctgatccttgttctcatgtttgtggtatggatgaaacggcgggataaagagcgccaagccaagcagcttttaattgacccagaagatgatgtaagagataatatattgaaatatgatgaagaaggtggaggagaagaagaccaggactatgacttgagccagctccagcaaccagatactgtggagcctgatgccatcaagcccgtgggaatcagacggctagacgagaggcctatccatgctgagccacagtacccagtccgatccgcagccccacaccctggggatattggggacttcattaatgagggccttaaagctgctgacaacgaccccacggcgccaccgtatgactccctcttagtctttgactacgagggcagcggctccacggctggctccttgagctccctcaactcctccagtagcggtggggaccaggactatgactacctgaatgactggggaccccgcttcaagaaactggcggacatgtacggcggtggtgacgacagtcactagtggatctggcgccaccaacttctctctgctgaagcaggccggcgacgtggaggagaacccaggcccaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511600_sequence 1 atgtgccggatagcgggagcgccgcggaccctgctgccgcttctggcggccttgcttcaggcgtctgtggaggcttctggtgaaattgcattatgcaagactggatttcctgaagatgtttacagcgcagtcttaccgaaggatgtgcacgaaggacagccccttctcaatgtgaaattcagcaactgcaatagaaaaaggaaagttcagtatgaaagcagcgagccagcagatttcaaggtggacgaggacggcacggtgtatgctgtgagaagcttccctctcactgcagagcaggcaaagttcctgatatatgcccaagacaaagaaacccaggaaaagtggcaggtagctgtaaacctgagccgggagccaaccctgactgaggagcctatgaaggaaccacatgaaattgaagaaatagtattccctagacaacttgccaagcacagtggagctctacaaaggcagaagagagactgggtcatcccgccaatcaacttgccagaaaactccagaggaccctttcctcaagagcttgtcagaatcaggtctgatagagataaaaacctttccctgagatacagcgtcactgggccaggagctgaccagcctccaacgggcatcttcattatcaaccccatctcaggacagctgtcagtcacaaagcctctggatcgagagctgatagcccggtttcacttgagagcacatgcagtggacatcaatggcaatcaagtggagaaccccattgacattgtcatcaatgttattgacatgaatgataacagacctgagtttctgcaccaggtttggaatgggtctgttccagagggatcaaagcctgggacgtatgtgatgacggtcactgccattgatgcggatgatccaaatgccctgaatggaatgctgcggtacaggatcctgtcccaggcgcccagcacaccttcacccaacatgtttacaatcaacaatgagactggggacatcatcactgtggcagctggtctggatcgagagaaagtgcaacagtatacgttaataattcaagccacagacatggaaggcaatcccacttatggcctttcaaacacagccacagccgtcatcacggtgacagatgtcaatgacaatcctccagagtttactgccatgactttctacggagaagtccctgagaacagggtggacgtcattgtagccaacctaactgtcacggacaaagatcagccccacacgccggcctggaatgcggcatacagaatcagtggtggagaccctacaggaaggtttgccatcctgacagaccccaacagcaatgatgggctagtcacagtggtaaaaccaattgactttgaaacgaataggatgtttgtccttactgttgctgcagaaaaccaagtgccattagctaaaggcattcagcacccacctcagtcgacagccactgtgtctgtgacagttattgatgtcaatgaaaatccttattttgccccaaatcctaaaatcattcgccaagaggaaggcctccacgcaggtaccatgctgaccacgctcactgctcaggaccccgatcgatatatgcaacagaatatcagatacacaaaattgtctgatcctgccaactggctgaaaatagaccccgtgaatgggcagatcactactattgccgttttggacagagaatcgccaaatgtaaaaaacaacatctataatgctaccttccttgcttctgacaatggaatcccgcctatgagtgggacaggaacactgcaaatctatttacttgatatcaatgacaacgcccctcaggtgttacctcaagaggcagagacctgtgaaactccagaacccaactcaattaacatcacagcacttgattatgacatagacccaaacgccgggccattcgcgtttgatcttcccttatctccagtgactattaaaagaaactggaccatcaaccggcttaatggtgattttgctcagctcaatttaaagataaaatttttggaagctggtatctatgaagttcccatcattatcacagattcagggaatccccccaagtccaacatttccatcctgcgtgtgaaagtttgccagtgtgactccaatggagactgcacggacgtggacaggatcgtgggtgcagggcttggcacgggtgccatcatcgctatccttctgtgtatcatcatcctgctgatccttgttctcatgtttgtggtatggatgaaacggcgggataaagagcgccaagccaagcagcttttaattgacccagaagatgatgtaagagataatatattgaaatatgatgaagaaggtggaggagaagaagaccaggactatgacttgagccagctccagcaaccagatactgtggagcctgatgccatcaagcccgtgggaatcagacggctagacgagaggcctatccatgctgagccacagtacccagtccgatccgcagccccacaccctggggatattggggacttcattaatgagggccttaaagctgctgacaacgaccccacggcgccaccgtatgactccctcttagtctttgactacgagggcagcggctccacggctggctccttgagctccctcaactcctccagtagcggtggggaccaggactatgactacctgaatgactggggaccccgcttcaagaaactggcggacatgtacggcggtggtgacgacagtcactagtggatctggcgccaccaacttctctctgctgaagcaggccggcgacgtggaggagaacccaggcccaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K511003_sequence 1 gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z