BBa_K511826
1
BBa_K511826
Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device
2011-09-27T11:00:00Z
2015-05-08T01:12:31Z
Consult constituent components.
This MammoBlock composite device produces both the N-Cadherin cell-cell adhesion compound and the yellow fluorescent protein EYFP when induced with Gal4 transactivator variants and otherwise produces both proteins at a low, basal (OFF) level. This generator is particularly well suited to the engineering of cellular patterns given the combination of adhesion and fluorescent readout.
false
false
_674_
0
6719
9
Not in stock
false
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.
false
Grant Robinson
component2146322
1
BBa_K511600
component2146316
1
BBa_K511003
component2146318
1
BBa_J85600
annotation2146318
1
BBa_J85600
range2146318
1
221
242
annotation2146322
1
BBa_K511600
range2146322
1
243
3756
annotation2146316
1
BBa_K511003
range2146316
1
1
220
BBa_J85600
1
BBa_J85600
attB1 recombination site
2011-09-05T11:00:00Z
2015-05-08T01:08:30Z
lambda phage
attB1 recombination site
Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning.
false
false
_406_
0
106
406
Not in stock
false
None
false
Ron Weiss
annotation2125966
1
attB1
range2125966
1
2
22
BBa_K511003
1
BBa_K511003
UAS-Gal4 Promoter MammoBlock
2011-09-24T11:00:00Z
2015-05-08T01:12:31Z
This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter.
This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise.
As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator.
false
false
_674_
0
6719
9
It's complicated
true
Not applicable.
false
Grant Robinson
annotation2139553
1
Major-Late Adenovirus Promoter
range2139553
1
160
200
annotation2139552
1
Gal4-Binding Region
range2139552
1
46
148
BBa_K511600
1
BBa_K511600
N-Cadherin-2A-EYFP Cell Adhesion MammoBlock
2011-09-26T11:00:00Z
2015-05-08T01:12:31Z
The N-Cadherin sequence of this part was isolated from a murine source; the viral 2A self-cleaving peptide was isolated from a virus with homology to the foot-and-mouth disease virus; the EYFP fluorescent reporter is an engineered version of the original green fluorescent protein from Aequorea victoria.
This part encodes a protein kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. This part additionally encodes an enhanced yellow fluorescent protein (EYFP) linked to the N-Cadherin by a self-cleaving viral 2A peptide, allowing for bicistronic expression of both a cell adhesion protein and an identifying marker with a single part.
false
false
_674_
0
6719
9
It's complicated
false
Not applicable.
false
Grant Robinson
annotation2144668
1
2A Self-Cleaving Peptide
range2144668
1
2719
2794
annotation2144667
1
Murine N-Cadherin
range2144667
1
1
2718
annotation2144670
1
EYFP
range2144670
1
2719
3511
BBa_K511826_sequence
1
gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgaccccaagtttgtacaaaaaagcagatgtgccggatagcgggagcgccgcggaccctgctgccgcttctggcggccttgcttcaggcgtctgtggaggcttctggtgaaattgcattatgcaagactggatttcctgaagatgtttacagcgcagtcttaccgaaggatgtgcacgaaggacagccccttctcaatgtgaaattcagcaactgcaatagaaaaaggaaagttcagtatgaaagcagcgagccagcagatttcaaggtggacgaggacggcacggtgtatgctgtgagaagcttccctctcactgcagagcaggcaaagttcctgatatatgcccaagacaaagaaacccaggaaaagtggcaggtagctgtaaacctgagccgggagccaaccctgactgaggagcctatgaaggaaccacatgaaattgaagaaatagtattccctagacaacttgccaagcacagtggagctctacaaaggcagaagagagactgggtcatcccgccaatcaacttgccagaaaactccagaggaccctttcctcaagagcttgtcagaatcaggtctgatagagataaaaacctttccctgagatacagcgtcactgggccaggagctgaccagcctccaacgggcatcttcattatcaaccccatctcaggacagctgtcagtcacaaagcctctggatcgagagctgatagcccggtttcacttgagagcacatgcagtggacatcaatggcaatcaagtggagaaccccattgacattgtcatcaatgttattgacatgaatgataacagacctgagtttctgcaccaggtttggaatgggtctgttccagagggatcaaagcctgggacgtatgtgatgacggtcactgccattgatgcggatgatccaaatgccctgaatggaatgctgcggtacaggatcctgtcccaggcgcccagcacaccttcacccaacatgtttacaatcaacaatgagactggggacatcatcactgtggcagctggtctggatcgagagaaagtgcaacagtatacgttaataattcaagccacagacatggaaggcaatcccacttatggcctttcaaacacagccacagccgtcatcacggtgacagatgtcaatgacaatcctccagagtttactgccatgactttctacggagaagtccctgagaacagggtggacgtcattgtagccaacctaactgtcacggacaaagatcagccccacacgccggcctggaatgcggcatacagaatcagtggtggagaccctacaggaaggtttgccatcctgacagaccccaacagcaatgatgggctagtcacagtggtaaaaccaattgactttgaaacgaataggatgtttgtccttactgttgctgcagaaaaccaagtgccattagctaaaggcattcagcacccacctcagtcgacagccactgtgtctgtgacagttattgatgtcaatgaaaatccttattttgccccaaatcctaaaatcattcgccaagaggaaggcctccacgcaggtaccatgctgaccacgctcactgctcaggaccccgatcgatatatgcaacagaatatcagatacacaaaattgtctgatcctgccaactggctgaaaatagaccccgtgaatgggcagatcactactattgccgttttggacagagaatcgccaaatgtaaaaaacaacatctataatgctaccttccttgcttctgacaatggaatcccgcctatgagtgggacaggaacactgcaaatctatttacttgatatcaatgacaacgcccctcaggtgttacctcaagaggcagagacctgtgaaactccagaacccaactcaattaacatcacagcacttgattatgacatagacccaaacgccgggccattcgcgtttgatcttcccttatctccagtgactattaaaagaaactggaccatcaaccggcttaatggtgattttgctcagctcaatttaaagataaaatttttggaagctggtatctatgaagttcccatcattatcacagattcagggaatccccccaagtccaacatttccatcctgcgtgtgaaagtttgccagtgtgactccaatggagactgcacggacgtggacaggatcgtgggtgcagggcttggcacgggtgccatcatcgctatccttctgtgtatcatcatcctgctgatccttgttctcatgtttgtggtatggatgaaacggcgggataaagagcgccaagccaagcagcttttaattgacccagaagatgatgtaagagataatatattgaaatatgatgaagaaggtggaggagaagaagaccaggactatgacttgagccagctccagcaaccagatactgtggagcctgatgccatcaagcccgtgggaatcagacggctagacgagaggcctatccatgctgagccacagtacccagtccgatccgcagccccacaccctggggatattggggacttcattaatgagggccttaaagctgctgacaacgaccccacggcgccaccgtatgactccctcttagtctttgactacgagggcagcggctccacggctggctccttgagctccctcaactcctccagtagcggtggggaccaggactatgactacctgaatgactggggaccccgcttcaagaaactggcggacatgtacggcggtggtgacgacagtcactagtggatctggcgccaccaacttctctctgctgaagcaggccggcgacgtggaggagaacccaggcccaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
BBa_J85600_sequence
1
ccaagtttgtacaaaaaagcag
BBa_K511600_sequence
1
atgtgccggatagcgggagcgccgcggaccctgctgccgcttctggcggccttgcttcaggcgtctgtggaggcttctggtgaaattgcattatgcaagactggatttcctgaagatgtttacagcgcagtcttaccgaaggatgtgcacgaaggacagccccttctcaatgtgaaattcagcaactgcaatagaaaaaggaaagttcagtatgaaagcagcgagccagcagatttcaaggtggacgaggacggcacggtgtatgctgtgagaagcttccctctcactgcagagcaggcaaagttcctgatatatgcccaagacaaagaaacccaggaaaagtggcaggtagctgtaaacctgagccgggagccaaccctgactgaggagcctatgaaggaaccacatgaaattgaagaaatagtattccctagacaacttgccaagcacagtggagctctacaaaggcagaagagagactgggtcatcccgccaatcaacttgccagaaaactccagaggaccctttcctcaagagcttgtcagaatcaggtctgatagagataaaaacctttccctgagatacagcgtcactgggccaggagctgaccagcctccaacgggcatcttcattatcaaccccatctcaggacagctgtcagtcacaaagcctctggatcgagagctgatagcccggtttcacttgagagcacatgcagtggacatcaatggcaatcaagtggagaaccccattgacattgtcatcaatgttattgacatgaatgataacagacctgagtttctgcaccaggtttggaatgggtctgttccagagggatcaaagcctgggacgtatgtgatgacggtcactgccattgatgcggatgatccaaatgccctgaatggaatgctgcggtacaggatcctgtcccaggcgcccagcacaccttcacccaacatgtttacaatcaacaatgagactggggacatcatcactgtggcagctggtctggatcgagagaaagtgcaacagtatacgttaataattcaagccacagacatggaaggcaatcccacttatggcctttcaaacacagccacagccgtcatcacggtgacagatgtcaatgacaatcctccagagtttactgccatgactttctacggagaagtccctgagaacagggtggacgtcattgtagccaacctaactgtcacggacaaagatcagccccacacgccggcctggaatgcggcatacagaatcagtggtggagaccctacaggaaggtttgccatcctgacagaccccaacagcaatgatgggctagtcacagtggtaaaaccaattgactttgaaacgaataggatgtttgtccttactgttgctgcagaaaaccaagtgccattagctaaaggcattcagcacccacctcagtcgacagccactgtgtctgtgacagttattgatgtcaatgaaaatccttattttgccccaaatcctaaaatcattcgccaagaggaaggcctccacgcaggtaccatgctgaccacgctcactgctcaggaccccgatcgatatatgcaacagaatatcagatacacaaaattgtctgatcctgccaactggctgaaaatagaccccgtgaatgggcagatcactactattgccgttttggacagagaatcgccaaatgtaaaaaacaacatctataatgctaccttccttgcttctgacaatggaatcccgcctatgagtgggacaggaacactgcaaatctatttacttgatatcaatgacaacgcccctcaggtgttacctcaagaggcagagacctgtgaaactccagaacccaactcaattaacatcacagcacttgattatgacatagacccaaacgccgggccattcgcgtttgatcttcccttatctccagtgactattaaaagaaactggaccatcaaccggcttaatggtgattttgctcagctcaatttaaagataaaatttttggaagctggtatctatgaagttcccatcattatcacagattcagggaatccccccaagtccaacatttccatcctgcgtgtgaaagtttgccagtgtgactccaatggagactgcacggacgtggacaggatcgtgggtgcagggcttggcacgggtgccatcatcgctatccttctgtgtatcatcatcctgctgatccttgttctcatgtttgtggtatggatgaaacggcgggataaagagcgccaagccaagcagcttttaattgacccagaagatgatgtaagagataatatattgaaatatgatgaagaaggtggaggagaagaagaccaggactatgacttgagccagctccagcaaccagatactgtggagcctgatgccatcaagcccgtgggaatcagacggctagacgagaggcctatccatgctgagccacagtacccagtccgatccgcagccccacaccctggggatattggggacttcattaatgagggccttaaagctgctgacaacgaccccacggcgccaccgtatgactccctcttagtctttgactacgagggcagcggctccacggctggctccttgagctccctcaactcctccagtagcggtggggaccaggactatgactacctgaatgactggggaccccgcttcaagaaactggcggacatgtacggcggtggtgacgacagtcactagtggatctggcgccaccaacttctctctgctgaagcaggccggcgacgtggaggagaacccaggcccaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
BBa_K511003_sequence
1
gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z