LOCUS       BBa_K515104              488 bp    DNA     linear       29-May-2024
DEFINITION  J23100 promoter - Antiholin
ACCESSION   BBa_K515104
VERSION     BBa_K515104.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    36..50
                     /label=insulator
     RBS             51..70
                     /label=rbs
     CDS             71..367
                     /label=antiholin
     misc_feature    36..367
                     /label=BBa_K515004
     stem_loop       379..422
                     /label=stem_loop
     misc_feature    368..447
                     /label=BBa_B0010
     terminator      368..447
                     /label=BBa_B0010
     stem_loop       455..474
                     /label=T7 TE
     misc_feature    481..481
                     /label=stop
     polyA_site      475..488
                     /label=polya
     misc_feature    448..488
                     /label=BBa_B0012
     terminator      448..488
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagccagcc tgcggtccgg gcgataataa
       61 ggaggtacta atggccttaa aagcaacagc actttttgcc atgctaggat tgtcatttgt
      121 tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta tggaatctgg
      181 tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg aacaattcta
      241 tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg aatgtataga
      301 gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat tggagactga
      361 ataataacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      421 atctgttgtt tgtcggtgaa cgctctctca cactggctca ccttcgggtg ggcctttctg
      481 cgtttata
//