LOCUS       BBa_K516020              972 bp    DNA     linear       14-May-2024
DEFINITION  AiiA protein generator TERM+ RBS+(B0030)
ACCESSION   BBa_K516020
VERSION     BBa_K516020.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start
     misc_feature    805..810
                     /label=2
     misc_feature    772..804
                     /label=LVA
     CDS             22..771
                     /label=aiiA
     misc_feature    22..810
                     /label=BBa_C0060
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             22..810
                     /label=BBa_C0060
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
     terminator      844..972
                     /label=BBa_B0015
ORIGIN
        1 attaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
       61 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
      121 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
      181 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
      241 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
      301 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
      361 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
      421 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
      481 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
      541 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
      601 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
      661 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
      721 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac
      781 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta ta
//