LOCUS       BBa_K516030              864 bp    DNA     linear       19-Apr-2024
DEFINITION  mRFP protein generator TERM+ RBS+(B0030)
ACCESSION   BBa_K516030
VERSION     BBa_K516030.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..696
                     /label=mrfp1
     misc_feature    703..727
                     /label=Help:Barcodes
     CDS             22..727
                     /label=BBa_E1010
     stem_loop       747..790
                     /label=stem_loop
     misc_feature    736..815
                     /label=BBa_B0010
     terminator      736..815
                     /label=BBa_B0010
     stem_loop       831..850
                     /label=T7 TE
     misc_feature    857..857
                     /label=stop
     polyA_site      851..864
                     /label=polya
     misc_feature    824..864
                     /label=BBa_B0012
     terminator      824..864
                     /label=BBa_B0012
     terminator      736..864
                     /label=BBa_B0015
ORIGIN
        1 attaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
       61 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      121 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      181 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      241 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      301 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      361 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      421 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      481 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      541 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      601 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      661 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      721 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      841 cgggtgggcc tttctgcgtt tata
//