LOCUS       BBa_K516031              863 bp    DNA     linear       07-May-2024
DEFINITION  mRFP protein generator TERM+ RBS+(B0031)
ACCESSION   BBa_K516031
VERSION     BBa_K516031.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             21..695
                     /label=mrfp1
     misc_feature    702..726
                     /label=Help:Barcodes
     CDS             21..726
                     /label=BBa_E1010
     stem_loop       746..789
                     /label=stem_loop
     misc_feature    735..814
                     /label=BBa_B0010
     terminator      735..814
                     /label=BBa_B0010
     stem_loop       830..849
                     /label=T7 TE
     misc_feature    856..856
                     /label=stop
     polyA_site      850..863
                     /label=polya
     misc_feature    823..863
                     /label=BBa_B0012
     terminator      823..863
                     /label=BBa_B0012
     terminator      735..863
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aacctactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
       61 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      121 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      181 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      241 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      301 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      361 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      421 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      481 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      541 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      601 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      661 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      721 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      781 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      841 gggtgggcct ttctgcgttt ata
//