LOCUS       BBa_K516130              907 bp    DNA     linear       14-May-2024
DEFINITION  Constitutive promoter [J23101] with mRFP, RBS B0030
ACCESSION   BBa_K516130
VERSION     BBa_K516130.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..739
                     /label=mrfp1
     misc_feature    746..770
                     /label=Help:Barcodes
     CDS             65..770
                     /label=BBa_E1010
     stem_loop       790..833
                     /label=stem_loop
     misc_feature    779..858
                     /label=BBa_B0010
     terminator      779..858
                     /label=BBa_B0010
     stem_loop       874..893
                     /label=T7 TE
     misc_feature    900..900
                     /label=stop
     polyA_site      894..907
                     /label=polya
     misc_feature    867..907
                     /label=BBa_B0012
     terminator      867..907
                     /label=BBa_B0012
     terminator      779..907
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
      121 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
      181 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
      241 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
      301 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
      361 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
      421 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
      481 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
      541 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
      601 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
      661 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
      721 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttata
//