LOCUS       BBa_K516131              906 bp    DNA     linear       15-May-2024
DEFINITION  Constitutive promoter [J23101] with mRFP, RBS B0031
ACCESSION   BBa_K516131
VERSION     BBa_K516131.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    50..53
                     /label=conserved
     RBS             44..57
                     /label=BBa_B0031
     CDS             64..738
                     /label=mrfp1
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             64..769
                     /label=BBa_E1010
     stem_loop       789..832
                     /label=stem_loop
     misc_feature    778..857
                     /label=BBa_B0010
     terminator      778..857
                     /label=BBa_B0010
     stem_loop       873..892
                     /label=T7 TE
     misc_feature    899..899
                     /label=stop
     polyA_site      893..906
                     /label=polya
     misc_feature    866..906
                     /label=BBa_B0012
     terminator      866..906
                     /label=BBa_B0012
     terminator      778..906
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaacctac
       61 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      121 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      181 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      241 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      301 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      361 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      421 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      481 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      541 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      601 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      661 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      721 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      901 tttata
//