LOCUS       BBa_K516220             1034 bp    DNA     linear       14-May-2024
DEFINITION  AiiA protein generator with Ptet and RBS B0030
ACCESSION   BBa_K516220
VERSION     BBa_K516220.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..86
                     /label=start
     misc_feature    867..872
                     /label=2
     misc_feature    834..866
                     /label=LVA
     CDS             84..833
                     /label=aiiA
     misc_feature    84..872
                     /label=BBa_C0060
     misc_feature    873..897
                     /label=Help:Barcodes
     CDS             84..872
                     /label=BBa_C0060
     stem_loop       917..960
                     /label=stem_loop
     misc_feature    906..985
                     /label=BBa_B0010
     terminator      906..985
                     /label=BBa_B0010
     stem_loop       1001..1020
                     /label=T7 TE
     misc_feature    1027..1027
                     /label=stop
     polyA_site      1021..1034
                     /label=polya
     misc_feature    994..1034
                     /label=BBa_B0012
     terminator      994..1034
                     /label=BBa_B0012
     terminator      906..1034
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgacag taaagaagct ttatttcgtc ccagcaggtc
      121 gttgtatgtt ggatcattcg tctgttaata gtacattaac accaggagaa ttattagact
      181 taccggtttg gtgttatctt ttggagactg aagaaggacc tattttagta gatacaggta
      241 tgccagaaag tgcagttaat aatgaaggtc tttttaacgg tacatttgtc gaagggcagg
      301 ttttaccgaa aatgactgaa gaagatagaa tcgtgaatat tttaaaacgg gttggttatg
      361 agccggaaga ccttctttat attattagtt ctcacttgca ttttgatcat gcaggaggaa
      421 atggcgcttt tataaataca ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc
      481 atagcgaaga atatttgaaa gaatgtatat tgccgaattt aaactacaaa atcattgaag
      541 gtgattatga agtcgtacca ggagttcaat tattgcatac accaggccat actccagggc
      601 atcaatcgct attaattgag acagaaaaat ccggtcctgt attattaacg attgatgcat
      661 cgtatacgaa agagaatttt gaaaatgaag tgccatttgc gggatttgat tcagaattag
      721 ctttatcttc aattaaacgt ttaaaagaag tggtgatgaa agagaagccg attgttttct
      781 ttggacatga tatagagcag gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa
      841 acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac
      901 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      961 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1021 tttctgcgtt tata
//