LOCUS       BBa_K516331             1840 bp    DNA     linear       15-May-2024
DEFINITION  mRFP producer controlled by 3OC6-HSL with RBS B0031
ACCESSION   BBa_K516331
VERSION     BBa_K516331.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     misc_feature    79..80
                     /label=prefix
     misc_feature    252..252
                     /label=T
     misc_feature    570..570
                     /label=A
     CDS             79..828
                     /label=luxR
     misc_feature    79..834
                     /label=BBa_C0062
     misc_feature    835..859
                     /label=Help:Barcodes
     CDS             79..834
                     /label=BBa_C0062
     misc_feature    868..906
                     /label=B1006
     stem_loop       877..898
                     /label=modified thr terminator
     polyA_site      899..906
                     /label=PolyA
     polyA_site      868..876
                     /label=PolyA
     terminator      868..906
                     /label=BBa_B1006
     misc_feature    915..934
                     /label=LuxR/HSL
     promoter        934..939
                     /label=-35
     promoter        956..961
                     /label=-10
     misc_feature    967..967
                     /label=start
     misc_feature    915..969
                     /label=BBa_R0062
     promoter        915..969
                     /label=BBa_R0062
     misc_feature    984..987
                     /label=conserved
     RBS             978..991
                     /label=BBa_B0031
     CDS             998..1672
                     /label=mrfp1
     misc_feature    1679..1703
                     /label=Help:Barcodes
     CDS             998..1703
                     /label=BBa_E1010
     stem_loop       1723..1766
                     /label=stem_loop
     misc_feature    1712..1791
                     /label=BBa_B0010
     terminator      1712..1791
                     /label=BBa_B0010
     stem_loop       1807..1826
                     /label=T7 TE
     misc_feature    1833..1833
                     /label=stop
     polyA_site      1827..1840
                     /label=polya
     misc_feature    1800..1840
                     /label=BBa_B0012
     terminator      1800..1840
                     /label=BBa_B0012
     terminator      1712..1840
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
      121 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      181 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      241 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      301 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      361 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      421 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      481 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      541 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      601 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      661 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      721 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      781 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      841 tagtgctagt gtagatcact actagagaaa aaaaaacccc gcccctgaca gggcggggtt
      901 tttttttact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
      961 tcgaataaat actagagtca cacaggaaac ctactagatg gcttcctccg aagacgttat
     1021 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1081 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1141 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1201 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1261 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     1321 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     1381 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     1441 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1501 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1561 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1621 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     1681 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
     1741 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1801 cacactggct caccttcggg tgggcctttc tgcgtttata
//