LOCUS       BBa_K516332             1839 bp    DNA     linear       13-May-2024
DEFINITION  mRFP producer controlled by 3OC6-HSL with RBS B0032
ACCESSION   BBa_K516332
VERSION     BBa_K516332.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     misc_feature    79..80
                     /label=prefix
     misc_feature    252..252
                     /label=T
     misc_feature    570..570
                     /label=A
     CDS             79..828
                     /label=luxR
     misc_feature    79..834
                     /label=BBa_C0062
     misc_feature    835..859
                     /label=Help:Barcodes
     CDS             79..834
                     /label=BBa_C0062
     misc_feature    868..906
                     /label=B1006
     stem_loop       877..898
                     /label=modified thr terminator
     polyA_site      899..906
                     /label=PolyA
     polyA_site      868..876
                     /label=PolyA
     terminator      868..906
                     /label=BBa_B1006
     misc_feature    915..934
                     /label=LuxR/HSL
     promoter        934..939
                     /label=-35
     promoter        956..961
                     /label=-10
     misc_feature    967..967
                     /label=start
     misc_feature    915..969
                     /label=BBa_R0062
     promoter        915..969
                     /label=BBa_R0062
     RBS             978..990
                     /label=RBS-3\Weak
     RBS             984..987
                     /label=RBS
     misc_feature    978..990
                     /label=BBa_B0032
     RBS             978..990
                     /label=BBa_B0032
     CDS             997..1671
                     /label=mrfp1
     misc_feature    1678..1702
                     /label=Help:Barcodes
     CDS             997..1702
                     /label=BBa_E1010
     stem_loop       1722..1765
                     /label=stem_loop
     misc_feature    1711..1790
                     /label=BBa_B0010
     terminator      1711..1790
                     /label=BBa_B0010
     stem_loop       1806..1825
                     /label=T7 TE
     misc_feature    1832..1832
                     /label=stop
     polyA_site      1826..1839
                     /label=polya
     misc_feature    1799..1839
                     /label=BBa_B0012
     terminator      1799..1839
                     /label=BBa_B0012
     terminator      1711..1839
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
      121 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      181 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      241 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      301 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      361 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      421 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      481 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      541 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      601 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      661 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      721 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      781 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      841 tagtgctagt gtagatcact actagagaaa aaaaaacccc gcccctgaca gggcggggtt
      901 tttttttact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
      961 tcgaataaat actagagtca cacaggaaag tactagatgg cttcctccga agacgttatc
     1021 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1081 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1141 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1201 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1261 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1321 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1381 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1441 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1501 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1561 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1621 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1681 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
     1741 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1801 acactggctc accttcgggt gggcctttct gcgtttata
//