LOCUS       BBa_K516334             1838 bp    DNA     linear       15-May-2024
DEFINITION  mRFP producer controlled by 3OC6-HSL with RBS B0034
ACCESSION   BBa_K516334
VERSION     BBa_K516334.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     misc_feature    79..80
                     /label=prefix
     misc_feature    252..252
                     /label=T
     misc_feature    570..570
                     /label=A
     CDS             79..828
                     /label=luxR
     misc_feature    79..834
                     /label=BBa_C0062
     misc_feature    835..859
                     /label=Help:Barcodes
     CDS             79..834
                     /label=BBa_C0062
     misc_feature    868..906
                     /label=B1006
     stem_loop       877..898
                     /label=modified thr terminator
     polyA_site      899..906
                     /label=PolyA
     polyA_site      868..876
                     /label=PolyA
     terminator      868..906
                     /label=BBa_B1006
     misc_feature    915..934
                     /label=LuxR/HSL
     promoter        934..939
                     /label=-35
     promoter        956..961
                     /label=-10
     misc_feature    967..967
                     /label=start
     misc_feature    915..969
                     /label=BBa_R0062
     promoter        915..969
                     /label=BBa_R0062
     misc_feature    982..985
                     /label=conserved
     RBS             978..989
                     /label=BBa_B0034
     CDS             996..1670
                     /label=mrfp1
     misc_feature    1677..1701
                     /label=Help:Barcodes
     CDS             996..1701
                     /label=BBa_E1010
     stem_loop       1721..1764
                     /label=stem_loop
     misc_feature    1710..1789
                     /label=BBa_B0010
     terminator      1710..1789
                     /label=BBa_B0010
     stem_loop       1805..1824
                     /label=T7 TE
     misc_feature    1831..1831
                     /label=stop
     polyA_site      1825..1838
                     /label=polya
     misc_feature    1798..1838
                     /label=BBa_B0012
     terminator      1798..1838
                     /label=BBa_B0012
     terminator      1710..1838
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
      121 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      181 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      241 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      301 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      361 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      421 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      481 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      541 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      601 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      661 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      721 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      781 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      841 tagtgctagt gtagatcact actagagaaa aaaaaacccc gcccctgaca gggcggggtt
      901 tttttttact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
      961 tcgaataaat actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
     1021 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     1081 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     1141 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     1201 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     1261 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     1321 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     1381 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     1441 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     1501 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1561 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1621 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     1681 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1741 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1801 cactggctca ccttcgggtg ggcctttctg cgtttata
//