LOCUS       BBa_K523014             2946 bp    DNA     linear       14-May-2024
DEFINITION  Plac + LacZ + bglX
ACCESSION   BBa_K523014
VERSION     BBa_K523014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..612
                     /label=BglII
     misc_feature    630..633
                     /label=Native RBS
     CDS             646..2946
                     /label=bglX
     misc_feature    646..646
                     /label=ATG
     misc_feature    2941..2941
                     /label=TAATAA
     misc_feature    693..693
                     /label=PstI site removed
     misc_feature    2730..2730
                     /label=G to T silent mutation
     misc_feature    646..705
                     /label=Predicted (cleaved) signal peptide
     misc_feature    609..2946
                     /label=BBa_K523002
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagat ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt
      661 tcagtaggaa tcgcggtgag tctggccctg caaccagcac tggcggatga tttattcggc
      721 aaccatccat taacgcccga agcgcgggat gcgttcgtca ccgaactgct taagaaaatg
      781 acagttgatg agaaaattgg tcagctgcgc ttaatcagcg tcggcccgga taacccgaaa
      841 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
      901 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
      961 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
     1021 ctggcctcgt cttttaacct cgatgcagtg aaaacggtcg gacgtgtctc tgcttatgaa
     1081 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
     1141 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aacaatgggt
     1201 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgctactc ggtgatgacc
     1261 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
     1321 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
     1381 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
     1441 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
     1501 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
     1561 gcggtgcgcg tggcgctgaa atccggaatc aacatgagca tgagcgacga gtactactcg
     1621 aagtatctgc ctgggttgat taaatccggc aaagtgacga tggcagagct ggacgatgct
     1681 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat
     1741 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
     1801 gaagcgcgtg aagtggcgcg cgaaagcttg gtgttgctga aaaaccgtct cgaaacgtta
     1861 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
     1921 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
     1981 attaaaaatg ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
     2041 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
     2101 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
     2161 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
     2221 actattccgc aaagccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
     2281 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
     2341 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
     2401 tttggcgatt acaacccgtc cggcaagctg ccaatgtcct tcccgcgttc tgtcgggcag
     2461 atcccggtgt actacagcca tctgaatacc ggtcgcccgt ataatgccga caagccgaac
     2521 aaatacactt cgcgttattt tgatgaagct aacggggcgt tgtatccgtt cggctatggg
     2581 ctgagctaca ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
     2641 gacggcaaag tgactgccag cgtgcaggtg acgaacaccg gtaagcgcga gggtgccacg
     2701 gtagtgcaga tgtacttgca ggatgtgact gcttccatga gtcgccctgt gaaacagctg
     2761 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
     2821 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
     2881 ttcaatgtct ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
     2941 taataa
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