LOCUS       BBa_K523016             2085 bp    DNA     linear       17-May-2024
DEFINITION  Plac + RBS + cex (exoglucanase)
ACCESSION   BBa_K523016
VERSION     BBa_K523016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     CDS             625..2082
                     /label=cex coding sequence
     misc_feature    1405..1410
                     /label=PstI site removed
     CDS             625..2085
                     /label=BBa_K118022
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatgcct aggaccacgc ccgcacccgg ccacccggcc
      661 cgcggcgccc gcaccgctct gcgcacgacg ctcgccgccg cggcggcgac gctcgtcgtc
      721 ggcgccacgg tcgtgctgcc cgcccaggcc gcgaccacgc tcaaggaggc cgccgacggc
      781 gccggccggg acttcggctt cgcgctcgac cccaaccggc tctcggaggc gcagtacaag
      841 gcgatcgccg acagcgagtt caacctcgtc gtcgccgaga acgcgatgaa gtgggacgcc
      901 accgagccct cgcagaacag cttctccttc ggcgcgggcg accgcgtcgc gagctacgcc
      961 gccgacaccg gcaaggagct gtacggccac acgctcgtat ggcactcgca gctgcccgac
     1021 tgggcgaaga acctcaacgg ctccgcgttc gagagcgcga tggtcaacca cgtgacgaag
     1081 gtcgccgacc acttcgaggg caaggtcgcg tcgtgggacg tcgtcaacga ggcgttcgcc
     1141 gacggcggcg gccgccggca ggactcggcg ttccagcaga agctcggcaa cggctacatc
     1201 gagaccgcgt tccgggcggc acgtgcggcg gacccgaccg ccaagctgtg catcaacgac
     1261 tacaacgtcg agggcatcaa cgcgaagagc aactcgctct acgacctcgt caaggacttc
     1321 aaggcgcgcg gcgtcccgct cgactgcgtc gggttccagt cgcacctcat cgtcggccag
     1381 gtgccgggcg acttccggca gaacctgcaa cggttcgcgg acctgggcgt ggacgtgcgc
     1441 atcaccgagc tcgacatccg catgcggacg ccctccgacg cgaccaagct cgcgacccag
     1501 gcggccgact acaagaaggt cgtgcaggcc tgcatgcagg tgacccgctg ccagggcgtg
     1561 accgtctggg gcatcaccga caagtactcg tgggtgccgg acgtcttccc gggcgagggg
     1621 gccgcgctgg tgtgggacgc gagctacgcc aagaagccgg cctacgccgc cgtgatggag
     1681 gccttcggcg cgagcccgac gccgacgccc accacgccga ccccgacgcc cacgacgccg
     1741 acgccgaccc cgacgtccgg tccggccggg tgccaggtgc tgtggggcgt caaccagtgg
     1801 aacaccggct tcaccgcgaa cgtcaccgtg aagaacacgt cctccgctcc ggtcgacggc
     1861 tggacgctca cgttcagctt cccgtccggc cagcaggtca cccaggcgtg gagctcgacg
     1921 gtcacgcagt ccggctcggc cgtgacggtc cgcaacgccc cgtggaacgg ctcgatcccg
     1981 gcgggcggca ccgcgcagtt cggcttcaac ggctcgcaca cgggcaccaa cgccgcgccg
     2041 acggcgttct cgctcaacgg cacgccctgc acggtcggct aataa
//