LOCUS       BBa_K523017             1248 bp    DNA     linear       17-May-2024
DEFINITION  Plac + RBS + rcsA
ACCESSION   BBa_K523017
VERSION     BBa_K523017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     CDS             625..1248
                     /label=BBa_K137113
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatgtca acgattatta tggatttatg tagttacacc
      661 cgactaggtt taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac
      721 attgaaaccg ttgatgacct tgccatagct tgtgattcac agcgcccttc agtggtgttt
      781 attaatgagg actgtttcat ccacgatgct tctaacagtc agcgtatcaa gctcatcatt
      841 aatcaacatc ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat
      901 gaatatctat tggtcagaaa aaatttattg atcagttcta aatcgattaa accggaatct
      961 ctcgacgata tccttggcga tattctgaaa aaagagacaa cgataacctc gtttttaaat
     1021 atgccgacgt tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt
     1081 cagggaacca ttcaaatctc tgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat
     1141 aaaggtaata ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc
     1201 cgactgacgg ataatgtgac taatggtatt tttgtcaaca tgcgctaa
//