LOCUS       BBa_K523018             2295 bp    DNA     linear       17-May-2024
DEFINITION  Plac + RBS + C. fimi β-glucosidase
ACCESSION   BBa_K523018
VERSION     BBa_K523018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     misc_feature    625..2295
                     /label=BBa_K392008
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatgggc gaccggttcc agcaggccgg tcgcccacgc
      661 cgccgcggcc cggcgagggc cgttaaccgt accggtcaag aagacgcgtc gacggggtcg
      721 agggagcggt cccacgcgtg tatcgtatcg tttcgacacc gccacccggc caccgggcac
      781 gcaccgggga cgcagcagtc cccgccccgg ccaccccctg tcaccgaaac ccgaaggacc
      841 ctcatgacca ccacgcgccc ctcgggccgc cagttctccg acgacttcct gtggggctcg
      901 gccacggcct cctaccagat cgagggcgcg cacgacgagg gcggtcgcgg cccgtcgatc
      961 tgggacacgt tctcccgcac cccgggcaag gtcctcaacg gcgacaccgg cgacgtcgcg
     1021 gtcgaccact accaccgcgt ccccgaggac gtcgagatca tgaagagcct cggcctccag
     1081 gcctaccggt tctcgatcgc ctggccccgc atccagccga ccggctcggg cgagttcaac
     1141 caggccgggc tcgacttcta ctccgacctc gtcgaccggc tcatcgccgc cggcatcaag
     1201 cccgtcgcga cgctgtacca ctgggacctc ccgcagcccc tggaggacga gggcggctgg
     1261 gcgaaccgcg cgacggcgta ccggttcgtc gagtacgcgc gcaagctcgc cgaggtcctc
     1321 ggcaagcgcg tcgacctgtg gaccacgctc aacgagccgt ggtgctccgc gttcctcggc
     1381 tacgcctcag gcgtgcacgc cccggcgtca ccgacccggt caaggccctg cgcgccgtcc
     1441 accacctcaa cctcgcgcac ggcctcgcgg gccgcgcgat ccgcgaggtc ctcggcgaga
     1501 acgcgcccgt ctcgatcacg tgaacctgca cgtgacgcgc gccgccgacg actccgccga
     1561 ctccgtcgag gccaagcgcc gcatcgacac catcgcgaac gaggtcttcc tcggcccgct
     1621 gctcgacggc gagtacccca aggaggtctt cgcggacacc gcgcaccaca ccgactggtc
     1681 cttcgtcgag ccgggcgacc tcgagctcat ccgcatcccg ctcaccgtgc tcggcgtcaa
     1741 ctactactcg accggccgcg tgaagaaggg cagcgcgcgg gcgccgaccc gggcaagccg
     1801 ggccccgacg gccaccgcgc ctccgagcac tcctcgtggg tcggcgccga cgaggtcgag
     1861 tggctcccgc agcccggccc gcacaccgcg atgggctgga acatcgagcc cgacggcctc
     1921 gtcgacctgc tgctcgagct gcgcgaccgt taccccagcc agcccctggc gatcacggag
     1981 aacggtgccg cgttctacga cacggtcagc gaggacggcc gcgtgcacga ccccgagcgc
     2041 gtcggctacc tgcacgacca cgtcgacgcc gtcggcgagg ccatcgacaa gggcgccgac
     2101 gtgcgcgcct acttcgtgtg gtcgctgctc gacaacttcg agtggcgcta cggctacgac
     2161 cgccggttcg gcatcgtgcg cgtcgactac gacacgcacg agcgcatcgt gaaggactcg
     2221 ggcctctggt accgcgagct cgtgcgcacc cgcaccatcg ctccggccga ggacgccgcg
     2281 acctaccagc cctga
//