LOCUS       BBa_K523027             1950 bp    DNA     linear       17-May-2024
DEFINITION  Plac + RBS + xylA (xylose isomerase)
ACCESSION   BBa_K523027
VERSION     BBa_K523027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     misc_feature    625..625
                     /label=ATG
     CDS             625..1950
                     /label=xylA
     misc_feature    1945..1945
                     /label=TAATAA
     CDS             625..1950
                     /label=BBa_K523026
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatgcaa gcctattttg accagctcga tcgcgttcgt
      661 tatgaaggct caaaatcctc aaacccgtta gcattccgtc actacaatcc cgacgaactg
      721 gtgttgggta agcgtatgga agagcacttg cgttttgccg cctgctactg gcacaccttc
      781 tgctggaacg gggcggatat gtttggtgtg ggggcgttta atcgtccgtg gcagcagcct
      841 ggtgaggcac tggcgttggc gaagcgtaaa gcagatgtcg catttgagtt tttccacaag
      901 ttacatgtgc cattttattg cttccacgat gtggatgttt cccctgaggg cgcgtcgtta
      961 aaagagtaca tcaataattt tgcgcaaatg gttgatgtcc tggcaggcaa gcaagaagag
     1021 agcggcgtga agctgctgtg gggaacggcc aactgcttta caaaccctcg ctacggcgcg
     1081 ggtgcggcga cgaacccaga tcctgaagtc ttcagctggg cggcaacgca agttgttaca
     1141 gcgatggaag caacccataa attgggcggt gaaaactatg tcctgtgggg cggtcgtgaa
     1201 ggttacgaaa cgctgttaaa taccgacttg cgtcaggagc gtgaacaact gggccgcttt
     1261 atgcagatgg tggttgagca taaacataaa atcggtttcc agggcacgtt gcttatcgaa
     1321 ccgaaaccgc aagaaccgac caaacatcaa tatgattacg atgccgcgac ggtctatggc
     1381 ttcctgaaac agtttggtct ggaaaaagag attaaactga acattgaagc taaccacgcg
     1441 acgctggcag gtcactcttt ccatcatgaa atagccaccg ccattgcgct tggcctgttc
     1501 ggttctgtcg acgccaaccg tggcgatgcg caactgggct gggacaccga ccagttcccg
     1561 aacagtgtgg aagagaatgc gctggtgatg tatgaaattc tcaaagcagg cggtttcacc
     1621 accggtggtc tgaacttcga tgccaaagta cgtcgtcaaa gtactgataa atatgatctg
     1681 ttttacggtc atatcggcgc gatggatacg atggcactgg cgctgaaaat tgcagcgcgc
     1741 atgattgaag atggcgagct ggataaacgc atcgcgcagc gttattccgg ctggaatagc
     1801 gaattgggcc agcaaatcct gaaaggccaa atgtcactgg cagatttagc caaatatgct
     1861 caggaacatc atttgtctcc ggtgcatcag agtggtcgcc aggaacaact ggaaaatctg
     1921 gtaaaccatt atctgttcga caaataataa
//