LOCUS BBa_K525007 3999 bp DNA linear 28-Apr-2024 DEFINITION Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB ACCESSION BBa_K525007 VERSION BBa_K525007.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 misc_feature 20..44 /label=lac operator misc_feature 53..64 /label=B0034 RBS 56..59 /label=RBS misc_feature 73..75 /label=ATG CDS 73..2400 /label=SgsE domain misc_feature 73..2400 /label=K525301 misc_feature 76..82 /label=NgoMIV misc_feature 2401..2406 /label=Freiburg Scar misc_feature 2407..3999 /label=K525515 CDS 2407..2718 /label=BisdA misc_feature 2719..2724 /label=Freiburg Scar CDS 2725..3999 /label=BisdB misc_feature 3991..3996 /label=AgeI misc_feature 3997..3999 /label=TAA ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctaccaag ctcgacaaga tgcgccagga gctgaaggca 121 gctgttgacg caaaggatct caagaaggct gaggaactgt accacaagat ctcctacgag 181 cttaagaccc gtactgttat cctcgaccgc gtctacggcc agtccacccg tgagctgctc 241 cgctccacct tcaaggcaga cgctcaggca ctgcgtgatt ctcttatcta cgacatcacc 301 gttgctatga aggcacgcga agctcaggac gcagttaagg ctggtaacct cgataaggca 361 aaggctgcac tggaccaggt caaccagtac gtttccaagg ttactgacgc tttcaaggca 421 gagctccaga aggctgcaca ggatgctaag gcagcttacg aggcagctct gccaccaaag 481 gtcgaatccg tgaccgcagt taacgctaag acccttgaga tcaagttcaa caaggcagtt 541 gacgctgcaa ccgtcatcga caacaagggc acttccgata cctctgacga cgttgttaag 601 gctaccgcaa tcaccctcaa ggctatcgat gaccaggtcc ctgtttccac tgttaaggca 661 tccctgtccg acgataagaa gaccctcaag ctggtcgtgg acggcgctca gttcttcacc 721 aagcgttacg ttgttgacat caagaacgtc aagacccttg atggtaagga cgttccagca 781 tacactacca ccatcgacac cactgattct gttcgcccat ccgtcctctc cttctcctac 841 gctgacaacg gcctgaccct caaggttaag ttctctgagc ctctggcatc cgttggcacc 901 gtcaagcttt acgacggtac cactgaaatc tccgtgtccc caaagttcac cgctggcgat 961 gacgagatga ccatcaacct cgcatcttcc tccgttccag ttaacaagga cctgaccctc 1021 aagatcttcg gcgctgtcga ttacaacggt aacgttatca accctaaccc agcagagctg 1081 actgttaaga agaccaccgt cgacatcacc aagccagttg ttcagtccat cgaagctgtc 1141 aacactaaga ccgtgaaggt taccttctct gagaagcttc tctccgcacc taccatcaag 1201 atcggcggcc agactgcttc cgtttccgtc gactctactg gtctggttta caccgcaacc 1261 ctcgcttccg cactgtccaa gggcgtttac gctgtcgagg tttccgatta caaggacctt 1321 gcaggcaact ctggtgacgc ttacactaag gttgtccagc tcaaggcaga taacaccgct 1381 ccaaagttcg tgtcctccca ggttgttaag atcaacggcg tcgaacacct ggttctcacc 1441 ttcgacgagg aggttaccac tggctccaac atcaccgtcg ttcagtcttc cgacaagtac 1501 atcgatgaaa acaacgttct gaaggcagtc ggtgctgacc ttaagaccac ctccgacaac 1561 ttcaagctct acctgccaac tgatggcaag tccaagtctg tggcactcaa catctcctcc 1621 ctgcctaagg gcacctacac cgttaccctt ccaaacggtc tcgtttccga cctggctgac 1681 aacccatacg cagagcgtaa gcagatcact ttcgtccgcg gctctgattc cctcaccacc 1741 aagcctgctc tggacaagga ctacgattcc aacggcgtta aggcagacaa caacaacgag 1801 cttgttttcg ctttcaccca gaacctcgac gcatccgctc tgaacctctc taacttcaac 1861 atcaacggtc tgactgtcac caaggcagtt ttcgatggcg acaccaagca catccgtgtt 1921 acccttgctc caggcgcaaa cacttggact ggtacccacg tcatcaccat ctccaacatc 1981 aagaacactt ccggcctcgt gatggacacc gttaccgtta acgaatacat gaaggagaac 2041 gtcgctccaa ccttcactgc aaccctgacc tccgctgatg ttatccgcgt tgacttctct 2101 gagcctgtcg caaacgctac catctcccgt gcactctccg ctaacaactt catcgttaag 2161 gttgacggca acgtcgtgac tgtttccaac gtttacgaag ataacaacgc aaccaacctg 2221 gtccagggtt ctaagggcta caagaccgtt taccttaagc tccagtcccc agttaccgac 2281 ctgtccaagc caatcactct ctccgctacc gacatcgtcg atgttaacca gaccggcgca 2341 atcaccgaca acaacgttgt cggtaacaac gtgtctgcta ctgttgttaa cgtcgcaaag 2401 accggccctc atatccaagt tacgactcgt gatggtgaaa ttcgcgagct ggacgttgca 2461 gcttccggtt tcctgatgga agcgctgcgt gacgctaaca tcgatggtgt ggaggcgatg 2521 tgtggcggtt gctgttcttg cgcgacttgc cacgtctaca ttgacgcggc tccagccggt 2581 accctgccgc cggtgtctag cgatgaagaa atgctgctgt ccggcctggt ttctaccccg 2641 ggccgctctc gtctgtcttg ccagatcccg gtaaccgcag aactggacgg cctgaaactg 2701 accatcccgc cggatagcac cggcaaccca caaactctgc cggtatttcc agacctggat 2761 atcttcagcc cggaatacgc ttgtaaccgc gaaaaatatg cagcgcgtgc gctgcgcgat 2821 tacccgctgc acttttataa accgctgaac ctgtggattg tctccaaaca taaagacgtt 2881 cgtagcgcgc tgttcacccc gcaggtcttc agctccgtag cttttggtct gctgcctcca 2941 ccagacgata tcgctcctcg cgtgccggac ctgtacaccg acgttcatct gccgtccatg 3001 gatccgccgg agcacaccaa actgcgtgtt ccggtgcagc aggctctgct gccgggccgt 3061 ctggtaggca aagacgaggt tgtgcgccgt attgcgaacg aactgatcga tacttttatc 3121 gacaaaggcg aatgcgatct gctgcatgat ttctcttata agctggcgct gtacctgatt 3181 gtagacctgc tgggtatccc taaagaacgt gcggaggact accaccgctg gtccaactgt 3241 ttcttccagc tgttcacgcc gaaggttcct gaacgtgcgg atgctcgttt ctttgtaccg 3301 atgccggagg aagtgctgcg ccagaactgg gaaggtctgg ccgaagcaaa tgactatctg 3361 cgtgaagttg ttgaaaacct ggaccgtaat ccgggcaaca acatgctgtc taacctgctg 3421 caactgcgtg aaccggatgg ctctcgtact atcactatct ccgcgaacgt ttgcaacgcc 3481 ctggaatttg gcgcggcggg tcacgatacg accgcaaccc tgatcgctca cctgacttat 3541 ttcgtgctga ccaccccgga cctgaaggac actctgaccg aagatccatc tctgattccg 3601 gcagccatct ctgaaaccct gcgtcgtcgt tgcccggttg acggtctgtt ccgccgcacc 3661 ctgagcgacg tagaactgtg cggtcagaag atcgaatccg gttctatcgt gtacctggac 3721 ctgaccgccg ctaacctgga tccggacgtt ttcccggagc cggagacttt ccgtctgaac 3781 cgcgataaca ttaaagaaat ggtgtctttc ggcttcggtc gtcacgtctg tgcaggtcag 3841 tacctgagcc gcatcgaagc taaagcagca tacgaagaac tgatgcgtcg tattcctaat 3901 atgcgtctgg cagatggctt caaactggag tacatgccga gcgtggccac cactgttctg 3961 aaaggcctgc cgctggtttg ggataaaaac accggttaa //