LOCUS BBa_K525009 4434 bp DNA linear 30-Apr-2024 DEFINITION Fusion Protein of S-Layer SgsE and LigA with His-Tag ACCESSION BBa_K525009 VERSION BBa_K525009.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 promotor misc_feature 1..2409 /label=K525303 misc_feature 20..44 /label=lac operator RBS 53..64 /label=B0034 misc_feature 73..75 /label=ATG CDS 73..2409 /label=SgsE domain misc_feature 76..82 /label=NogMIV misc_feature 2401..2407 /label=Freiburg scar misc_feature 2410..4434 /label=K525740 CDS 2407..4416 /label=LigA domain misc_feature 4417..4434 /label=His-Tag ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctaccaag ctcgacaaga tgcgccagga gctgaaggca 121 gctgttgacg caaaggatct caagaaggct gaggaactgt accacaagat ctcctacgag 181 cttaagaccc gtactgttat cctcgaccgc gtctacggcc agtccacccg tgagctgctc 241 cgctccacct tcaaggcaga cgctcaggca ctgcgtgatt ctcttatcta cgacatcacc 301 gttgctatga aggcacgcga agctcaggac gcagttaagg ctggtaacct cgataaggca 361 aaggctgcac tggaccaggt caaccagtac gtttccaagg ttactgacgc tttcaaggca 421 gagctccaga aggctgcaca ggatgctaag gcagcttacg aggcagctct gccaccaaag 481 gtcgaatccg tgaccgcagt taacgctaag acccttgaga tcaagttcaa caaggcagtt 541 gacgctgcaa ccgtcatcga caacaagggc acttccgata cctctgacga cgttgttaag 601 gctaccgcaa tcaccctcaa ggctatcgat gaccaggtcc ctgtttccac tgttaaggca 661 tccctgtccg acgataagaa gaccctcaag ctggtcgtgg acggcgctca gttcttcacc 721 aagcgttacg ttgttgacat caagaacgtc aagacccttg atggtaagga cgttccagca 781 tacactacca ccatcgacac cactgattct gttcgcccat ccgtcctctc cttctcctac 841 gctgacaacg gcctgaccct caaggttaag ttctctgagc ctctggcatc cgttggcacc 901 gtcaagcttt acgacggtac cactgaaatc tccgtgtccc caaagttcac cgctggcgat 961 gacgagatga ccatcaacct cgcatcttcc tccgttccag ttaacaagga cctgaccctc 1021 aagatcttcg gcgctgtcga ttacaacggt aacgttatca accctaaccc agcagagctg 1081 actgttaaga agaccaccgt cgacatcacc aagccagttg ttcagtccat cgaagctgtc 1141 aacactaaga ccgtgaaggt taccttctct gagaagcttc tctccgcacc taccatcaag 1201 atcggcggcc agactgcttc cgtttccgtc gactctactg gtctggttta caccgcaacc 1261 ctcgcttccg cactgtccaa gggcgtttac gctgtcgagg tttccgatta caaggacctt 1321 gcaggcaact ctggtgacgc ttacactaag gttgtccagc tcaaggcaga taacaccgct 1381 ccaaagttcg tgtcctccca ggttgttaag atcaacggcg tcgaacacct ggttctcacc 1441 ttcgacgagg aggttaccac tggctccaac atcaccgtcg ttcagtcttc cgacaagtac 1501 atcgatgaaa acaacgttct gaaggcagtc ggtgctgacc ttaagaccac ctccgacaac 1561 ttcaagctct acctgccaac tgatggcaag tccaagtctg tggcactcaa catctcctcc 1621 ctgcctaagg gcacctacac cgttaccctt ccaaacggtc tcgtttccga cctggctgac 1681 aacccatacg cagagcgtaa gcagatcact ttcgtccgcg gctctgattc cctcaccacc 1741 aagcctgctc tggacaagga ctacgattcc aacggcgtta aggcagacaa caacaacgag 1801 cttgttttcg ctttcaccca gaacctcgac gcatccgctc tgaacctctc taacttcaac 1861 atcaacggtc tgactgtcac caaggcagtt ttcgatggcg acaccaagca catccgtgtt 1921 acccttgctc caggcgcaaa cacttggact ggtacccacg tcatcaccat ctccaacatc 1981 aagaacactt ccggcctcgt gatggacacc gttaccgtta acgaatacat gaaggagaac 2041 gtcgctccaa ccttcactgc aaccctgacc tccgctgatg ttatccgcgt tgacttctct 2101 gagcctgtcg caaacgctac catctcccgt gcactctccg ctaacaactt catcgttaag 2161 gttgacggca acgtcgtgac tgtttccaac gtttacgaag ataacaacgc aaccaacctg 2221 gtccagggtt ctaagggcta caagaccgtt taccttaagc tccagtcccc agttaccgac 2281 ctgtccaagc caatcactct ctccgctacc gacatcgtcg atgttaacca gaccggcgca 2341 atcaccgaca acaacgttgt cggtaacaac gtgtctgcta ctgttgttaa cgtcgcaaag 2401 accggcgaat caatcgaaca acaactgaca gaactgcgaa cgacgcttcg ccatcatgaa 2461 tatctttatc atgtgatgga tgcgccggaa attcccgacg ctgaatacga caggctgatg 2521 cgcgaactgc gcgagctgga aaccaaacat ccagaactga ttacgcctga ttcgcctact 2581 caacgtgtag gcgctgcgcc gctggcggct ttcagccaga tacgccatga agtaccaatg 2641 ctgtcactgg ataacgtttt tgatgaagaa agctttcttg ctttcaacaa acgtgtgcag 2701 gaccgtctga aaaacaacga gaaagtcacc tggtgctgtg agctgaagct ggatggtctt 2761 gccgtcagta ttctgtatga aaatggcgtt ttagtcagtg ccgcgacccg tggcgatggc 2821 accaccgggg aagatatcac gtctaatgtg cgtactattc gcgccattcc gctgaagctg 2881 cacggagaga atatcccggc gcgtctggaa gtgcgtggtg aagtgttcct gccgcaggcg 2941 gggttcgaaa agattaacga agatgcgcga cgcacgggcg ggaaagtgtt tgctaaccca 3001 cgtaatgcgg cagctggttc actgcgtcag cttgatccgc gtattacagc gaagcgaccg 3061 ctcacttttt tctgctatgg cgttggtgtt ctggaaggtg gcgagctgcc ggatactcat 3121 cttggccgtt tactgcaatt taaaaagtgg gggttgccgg tcagcgatcg ggtaacgctt 3181 tgtgaatcgg cggaagaagt gctggcgttc tatcacaaag tggaagaaga ccgcccgacg 3241 ctgggctttg atatcgacgg cgtggtgatt aaggtcaact cactggcaca gcaggagcag 3301 cttggctttg tcgcgcgtgc cccgcgctgg gcggtagcgt ttaaattccc ggcgcaggag 3361 cagatgacct ttgtgcgtga cgtcgagttt caggttgggc gtactggcgc gattacgcct 3421 gttgcgcgtc tggaacctgt ccatgttgca ggcgtgctgg tgagtaacgc aaccttacac 3481 aatgcggatg aaatcgaacg tcttggttta cgcattggcg ataaagtggt gattcgccgc 3541 gctggcgacg tgatcccgca ggtggttaac gtcgtgcttt ctgaacgccc ggaagatacc 3601 cgtgaggttg tattcccgac gcattgtccg gtatgtggtt ctgacgttga gcgtgtggaa 3661 ggtgaagcgg ttgcccgctg taccggcggc ctgatttgcg gtgcgcagcg taaagagtcg 3721 ctgaaacact ttgtttcccg ccgtgcgatg gatgttgacg gaatgggcga caaaatcatc 3781 gatcagctgg ttgaaaaaga atatgtccat actccggcag atctgttcaa actcaccgca 3841 ggcaaactga ccggactgga gcgtatgggg ccaaaatcgg cacaaaacgt ggttaacgcg 3901 ctggaaaaag cgaaagaaac cacctttgct cgcttcctct atgcacttgg catccgtgaa 3961 gtcggcgagg ccaccgcagc aggtctggcg gcatatttcg gcacgctgga agcgctggaa 4021 gccgcttcga ttgaagagct gcaaaaggtg cctgatgttg gcattgtcgt tgcatcccac 4081 gttcacaact tctttgccga agaaagcaac cgcaatgtca tcagcgagct gttggcggaa 4141 ggtgttcact ggcctgcgcc gatcgttatc aacgcggaag agattgacag cccgtttgct 4201 ggtaaaaccg tggtgcttac gggcagctta agccagatgt cgcgtgatga cgctaaagct 4261 cgactggtcg aactgggcgc gaaagtcgcg ggcagcgtgt cgaagaaaac cgatctggtg 4321 atagcgggtg aagcggcagg atctaaactg gcgaaggcgc aggaactggg cattgaagtc 4381 atcgacgaag cggaaatgct gcgtttgctg ggtagccatc atcatcatca tcat //