Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Wittchen
Date created: 2011-10-24 11:00:00
Date modified: 2015-05-08 01:12:34
Fusion Protein of S-Layer SbpA and LigA with His-Tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K525010_sequence (Version 1) |
Description
Fusion Protein of S-Layer SbpA and LigA with His-TagNotes
Assembled with Freiburg Standard Assembly (RFC 25)Source
SbpA was synthesized, LigA is the native ligase of E. coli.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promotor lac operator B0034 ATG Freiburg scar SbpA domain LigA domain His-Tag K525403 K525740 NgoMIV | 1,20 20,44 53,64 73,75 3193,3199 73,3202 3199,5208 5209,5226 1,3193 3189,5226 76,81 | feature/promoter promoter feature/operator operator ribosome_entry_site feature/rbs start_codon feature/start sequence_feature feature/misc CDS feature/protein feature/protein CDS tag feature/tag feature/BioBrick engineered_region feature/BioBrick engineered_region feature/binding non_covalent_binding_site |