LOCUS       BBa_K525552             2466 bp    DNA     linear       03-May-2024
DEFINITION  Polycistronic expression of FNR, BisdA and BisdB
ACCESSION   BBa_K525552
VERSION     BBa_K525552.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     misc_feature    42..800
                     /label=K525499
     misc_feature    42..44
                     /label=ATG
     CDS             42..800
                     /label=Reductase
     misc_feature    45..50
                     /label=NgoMIV
     misc_feature    792..797
                     /label=AgeI
     misc_feature    798..800
                     /label=TAA
     misc_feature    809..820
                     /label=B0034
     RBS             812..815
                     /label=RBS
     misc_feature    827..1156
                     /label=K123000
     misc_feature    827..829
                     /label=ATG
     CDS             827..1156
                     /label=BisdA
     misc_feature    830..835
                     /label=NgoMIV
     misc_feature    1148..1153
                     /label=AgeI
     misc_feature    1154..1156
                     /label=TAA
     misc_feature    1165..1176
                     /label=B0034
     RBS             1168..1171
                     /label=RBS
     misc_feature    1183..1185
                     /label=ATG
     misc_feature    1183..2466
                     /label=K123001
     CDS             1183..2466
                     /label=BisdB
     misc_feature    1186..1191
                     /label=NgoMIV
     misc_feature    2458..2463
                     /label=AgeI
     misc_feature    2464..2466
                     /label=TAA
     misc_feature    42..2466
                     /label=BBa_K525551
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gatggccggc gctgattggg
       61 taacaggcaa agtcactaaa gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc
      121 acgcccccgt gcttccgttt accgccgggc aatttaccaa gcttggcctt gaaatcgacg
      181 gcgaacgcgt ccagcgcgcc tactcctatg taaactcgcc cgataatccc gatctggagt
      241 tttacctggt caccgtcccc gatggcaaat taagcccacg actggcggca ctgaaaccag
      301 gcgatgaagt gcaggtggtt agcgaagcgg caggattctt tgtgctcgat gaagtgccgc
      361 actgcgaaac gctatggatg ctggcaacgg gcacagcgat tggcccttat ttatcgattc
      421 tgcaactagg taaagattta gatcgcttca aaaatctggt cctggtgcac gccgcacgtt
      481 atgccgccga cttaagctat ttgccactga tgcaggaact ggaaaaacgc tacgaaggaa
      541 aactgcgcat tcagacggtg gtcagtcggg aaacggcagc ggggtcgctc accggacgga
      601 taccggcatt aattgaaagt ggggaactgg aaagcacgat tggcctgccg atgaataaag
      661 aaaccagcca tgtgatgctg tgcggcaatc cacagatggt gcgcgataca caacagttgc
      721 tgaaagagac ccggcagatg acgaaacatt tacgtcgccg accgggccat atgacagcgg
      781 agcattactg gaccggttaa tactagagaa agaggagaaa tactagatgg ccggccctca
      841 tatccaagtt acgactcgtg atggtgaaat tcgcgagctg gacgttgcag cttccggttt
      901 cctgatggaa gcgctgcgtg acgctaacat cgatggtgtg gaggcgatgt gtggcggttg
      961 ctgttcttgc gcgacttgcc acgtctacat tgacgcggct ccagccggta ccctgccgcc
     1021 ggtgtctagc gatgaagaaa tgctgctgtc cggcctggtt tctaccccgg gccgctctcg
     1081 tctgtcttgc cagatcccgg taaccgcaga actggacggc ctgaaactga ccatcccgcc
     1141 ggatagcacc ggttaatact agagaaagag gagaaatact agatggccgg caacccacaa
     1201 actctgccgg tatttccaga cctggatatc ttcagcccgg aatacgcttg taaccgcgaa
     1261 aaatatgcag cgcgtgcgct gcgcgattac ccgctgcact tttataaacc gctgaacctg
     1321 tggattgtct ccaaacataa agacgttcgt agcgcgctgt tcaccccgca ggtcttcagc
     1381 tccgtagctt ttggtctgct gcctccacca gacgatatcg ctcctcgcgt gccggacctg
     1441 tacaccgacg ttcatctgcc gtccatggat ccgccggagc acaccaaact gcgtgttccg
     1501 gtgcagcagg ctctgctgcc gggccgtctg gtaggcaaag acgaggttgt gcgccgtatt
     1561 gcgaacgaac tgatcgatac ttttatcgac aaaggcgaat gcgatctgct gcatgatttc
     1621 tcttataagc tggcgctgta cctgattgta gacctgctgg gtatccctaa agaacgtgcg
     1681 gaggactacc accgctggtc caactgtttc ttccagctgt tcacgccgaa ggttcctgaa
     1741 cgtgcggatg ctcgtttctt tgtaccgatg ccggaggaag tgctgcgcca gaactgggaa
     1801 ggtctggccg aagcaaatga ctatctgcgt gaagttgttg aaaacctgga ccgtaatccg
     1861 ggcaacaaca tgctgtctaa cctgctgcaa ctgcgtgaac cggatggctc tcgtactatc
     1921 actatctccg cgaacgtttg caacgccctg gaatttggcg cggcgggtca cgatacgacc
     1981 gcaaccctga tcgctcacct gacttatttc gtgctgacca ccccggacct gaaggacact
     2041 ctgaccgaag atccatctct gattccggca gccatctctg aaaccctgcg tcgtcgttgc
     2101 ccggttgacg gtctgttccg ccgcaccctg agcgacgtag aactgtgcgg tcagaagatc
     2161 gaatccggtt ctatcgtgta cctggacctg accgccgcta acctggatcc ggacgttttc
     2221 ccggagccgg agactttccg tctgaaccgc gataacatta aagaaatggt gtctttcggc
     2281 ttcggtcgtc acgtctgtgc aggtcagtac ctgagccgca tcgaagctaa agcagcatac
     2341 gaagaactga tgcgtcgtat tcctaatatg cgtctggcag atggcttcaa actggagtac
     2401 atgccgagcg tggccaccac tgttctgaaa ggcctgccgc tggtttggga taaaaacacc
     2461 ggttaa
//